• 专利标题:   Extracting target nucleic acid using magnetic nanoparticles, by separating the nucleic acid-magnetic nanoparticles-graphene oxide complex by applying magnetic field to mixture, removing impurities, and adding separation solution to complex.
  • 专利号:   KR2021132375-A
  • 发明人:   PARK J H
  • 专利权人:   HYBRID TECHNOLOGY INC
  • 国际专利分类:   C12N015/10, C01B032/198
  • 专利详细信息:   KR2021132375-A 04 Nov 2021 C12N-015/10 202194 Pages: 12
  • 申请详细信息:   KR2021132375-A KR050663 27 Apr 2020
  • 优先权号:   KR050663

▎ 摘  要

NOVELTY - Extracting a target nucleic acid using magnetic nanoparticles, involves: (i) mixing and reacting a sample containing the target nucleic acid and the magnetic nanoparticles-graphene oxide complex; (ii) separating the nucleic acid-magnetic nanoparticles-graphene oxide complex by applying a magnetic field to the mixture of step (i); (iii) removing impurities attached to the nucleic acid-magnetic nanoparticles-graphene oxide complex of step (ii); (iv) adding a separation solution to the nucleic acid-magnetic nanoparticle-graphene oxide complex from which the impurities of step (iii) have been removed so that the nucleic acid is dissolved from the magnetic nanoparticle-graphene oxide complex into a separation solution; and (v) removing the magnetic nanoparticles-graphene oxide complex by applying a magnetic field to the mixture of step (iv). USE - The method is useful for extracting target nucleic acid using magnetic nanoparticles (claimed). ADVANTAGE - The nucleic acid extraction method based on the magnetic nanoparticles-graphene oxide complex can extract nucleic acids such as RNA or DNA from various samples in inexpensive and convenient way. The method has simple manufacturing process. The stability of the composite is improved.