▎ 摘　　要

NOVELTY - Constructing electrochemical immunosensor comprises dropping the TiMOF/Ketjenblack (Electro-conductive carbon black) (KB) solution onto surface of a clean glassy carbon electrode, drying at room temperature, adding the gold nanoparticles (AuNPs) solution into the surface of the electrode, drying at room temperature, adding Ab1on the electrode, incubating overnight in the refrigerator at 4-5℃ for 12-13 hours, rinsing with diethyl pyrocarbonate water, adding 1% bovine serum albumin (BSA) solution, incubating at 4-5℃ for 30-35 minutes, rinsing the obtained electrode with diethylpyrocarbonate water, dropping polydiene dimethyl ammonium chloride (PDDA)- nitrogen-doped graphene (NG)/halloysite nano-tube (HNT)/TB-AuNPs/Ab2signal probe solution, and incubating at room temperature for 4-5 hours to obtain electrochemical immunosensor for the detection of zearalenone (ZEA). USE - The method is useful for constructing electrochemical immunosensor, which is useful for the detection of zearalenone (ZEA). ADVANTAGE - The method improves conductive activity of the electrode, increases specific surface area of the electrode, and combines signal probe with Ab1AgAb2to construct a sandwich sensor to achieve highly sensitive detection of zearalenone. The detection method has wide linear range, strong specificity, short analysis time, good stability and reproducibility, and is suitable for application in many fields. DETAILED DESCRIPTION - Constructing electrochemical immunosensor comprises dropping the TiMOF/Ketjenblack (Electro-conductive carbon black) (KB) solution onto surface of a clean glassy carbon electrode, drying at room temperature, adding the gold nanoparticles (AuNPs) solution into the surface of the electrode, drying at room temperature, adding Ab1on the electrode, incubating overnight in the refrigerator at 4-5℃ for 12-13 hours rinsing with diethyl pyrocarbonate water, adding 1% bovine serum albumin (BSA) solution, incubating at 4-5℃ for 30-35 minutes, rinsing the obtained electrode with diethylpyrocarbonate water, dropping polydiene dimethyl ammonium chloride (PDDA)- nitrogen-doped graphene (NG)/halloysite nano-tube (HNT)/TB-AuNPs/Ab2signal probe solution, and incubating at room temperature for 4-5 hours to obtain electrochemical immunosensor for the detection of zearalenone (ZEA). The preparation method of the PDDANG/HNT/TB-AuNPs/Ab2signal probe solution is as follows: adding the zearalenone polyclonal antibody (Ab2) into the prepared PDDANG/HNT/TB-AuNPs dispersion, stirring in an ice bath for 12-13 hours, centrifuging, washing with water, and redispersing the precipitate in ultrapure water to obtain PDDANG/HNT/TB-AuNPs/Ab2signal probe solution. The preparation method of the PDDANG/HNT/TB-AuNPs dispersion is as follows: adding AuNPs into the prepared PDDA-NG/HNT/TB dispersion, stirring for 4-5 hours, centrifuging, washing, and dispersing the precipitate in ultrapure water to obtain PDDA-NG/HNT/TB-AuNPs dispersion. The preparation method of the PDDANG/HNT/TB dispersion is as follows: adding toluidine blue (TB) into the obtained PDDANG/HNT dispersion, stirring for 12-13 hours, centrifuging and washing, and dispersing the precipitate in ultrapure water to obtain PDDANG/HNT/TB dispersion. The preparation method of the PDDA-NG/HNT dispersion liquid is as follows: dissolving nitrogen-doped graphene (NG) in N,N-dimethylformamide, adding polydiene dimethyl ammonium chloride (PDDA), stirring for 2-3 hours to obtain solution, adding halloysite nanotube (HNT), and stirring for 3 hours to obtain PDDA-NG/HNT dispersion liquid. The preparation method of the Ti-MOF/KB dispersion liquid is as follows: adding 2-aminoterephthalic acid (NH2-BDC) into N,N-dimethylformamide-methanol mixed solvent, adding titanic acid Tetrabutyl Ester and Ketjenblack (Electro-conductive carbon black)(KB), ultrasonicating at room temperature for 10-15 minutes, transforming the solution to a reaction kettle at 150-160℃ for 20-24 hours, cooling, and centrifuging at 10000 revolutions/minute for 5-6 minutes, washing with dimethylformamide and methanol, drying in an oven at 60-70℃ to obtain dark green Ti-MOF/KB nanomaterial, dissolving with ultrapure water to obtain Ti-MOF/KB dispersion. An INDEPENDENT CLAIM is also included for using method of the electrochemical immunosensor to detect zearalenone, comprising (A) dropping different concentrations of target zearalenone onto the sensor electrodes, (B) placing the electrode in 0.1 M phosphate buffered saline (pH = 7) solution for characterization, and measuring the electrochemical signal, (C) drawing a working curve according to the linear relationship between the current value and logarithmic value of the concentration of zearalenone, and (D) detecting the sample to be tested by the sensor, and calculating the current value through the working curve to obtain concentration of zearalenone in the sample to be tested.