▎ 摘　　要

NOVELTY - The method involves preparing nitrogen-doped graphene nanoparticles and aminated Golgi protein 73 aptamer (GP73Apt) of fluorescent resonance donor NGQDs-GP73apt, activating NGQD by EDC, mixing the two in the oscillation culture box for incubation, obtaining NGQDS-GP 73Apt solution, constructing the reaction system for fluorescence resonant energy transfer, adding graphene powder, adding ultra-pure water for constant volume, crushing, adding anti-cyclic blood acid, stirring and centrifuging, taking the precipitate, drying to obtain magnesium sulfate-RGO powder, mixing the magnesium sulfide-R GO solution and the NGQDD- USE - Method for fluorescence resonance energy transfer of non-diagnostic target nitrogen-doped graphene quantum dot and molybdenum disulfide. ADVANTAGE - The method has convenient detection, low cost, and satisfies detection standard. The method can reach the detection limit of 4.54 ng/ml. DESCRIPTION OF DRAWING(S) - The drawing schematic representation of a method for fluorescence resonance energy transfer of non-diagnostic target nitrogen-doped graphene quantum dot and molybdenum disulfide based on NGQDs-MoS2 @RGO of the fluorescence sensor for detecting the GP73.