▎ 摘　　要

NOVELTY - Preparing a carbon-rich chromatography medium involves mixing graphene oxide and agarose at a mass ratio of 0.01:99.99-10:90, dispersing them in water, formulating into an aqueous solution with a solid content of 2-15%, stirring and heating to a sol temperature of 60-98 degrees C to form an aqueous sol state. The water phase sol state is formed, stirred at constant temperature for 30 minutes, and then slowly lowered the temperature to 70 degrees C; The aqueous phase sol is dispersed into an oil phase containing emulsifiers at a temperature of 70 degrees C, mixed, stirred well at 250 rpm for 20 minutes. The temperature is reduces to room temperature to obtain microspheres containing carbon-rich components. The carbon-rich component microspheres is added to a cross-linking agent for cross-linking in the pH range of 7-12, and a carbon-rich chromatography medium is obtained. The carbon-rich chromatography medium is stored in a 20% ethanol aqueous solution. USE - Method for preparing a carbon-rich chromatography medium. ADVANTAGE - The method enables to prepare a carbon-rich chromatography medium enhances the mechanical properties of the chromatographic medium, provides good purification and separation effects, and has a certain light-driven self-repair capability.