▎ 摘　　要

NOVELTY - Detecting polymerase incorporation of nucleotides involves hybridizing test primers to immobilized primers; where the immobilized primers comprise a predetermined sequence of nucleotides and are attached to a substrate through their 5-prime ends, individual test primers are complementary to a portion of each of at least some of the immobilized primers, and no more than one test primer molecule hybridizes to an immobilized primer molecule, extending, using one nucleotide, at least some of the test primers with a polymerase according to templates; where the templates comprise immobilized primers hybridized to at least some of the test primers, and nucleotides incorporated into at least some of the test primers by the extending comprise one of fluorescent tags; and detecting an amount of fluorescent test primers. USE - The method is useful for detecting polymerase incorporation of nucleotides (claimed). ADVANTAGE - The method can measure on-surface extension by a polymerase of primers immobilized on a surface without requiring covalent modification of the immobilized primers, to permit subsequent use in sequencing by synthesis (SBS) methodology.