▎ 摘　　要

NOVELTY - Ionic liquid functionalized graphene arc Vibrio deoxyribonucleic acid electrochemistry sensor is claimed. The electrochemistry sensor is prepared by Vibrio deoxyribonucleic acid electrochemistry sensor uses a gold electrode as a base electrode, an ionic liquid functionalized graphene-ZIF-8 composite membrane as an electrode modification material, and a probe single-stranded deoxyribonucleic acid immobilized on the surface of the modified electrode by covalent bonding of the bioligand 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and N-hydroxysuccinimide; and the ionic liquid functionalized graphene-ZIF-8 complex is prepared by first preparing ZIF-8 in a graphene oxide dispersion, and covalently modifying graphene-ZIF-8 complex with an amino functionalized ionic liquid to obtain ionic liquid functionalized graphene-ZIF-8 complex. USE - The ionic liquid functionalized graphene arc Vibrio deoxyribonucleic acid electrochemistry sensor is useful for detecting Vibrio (claimed). ADVANTAGE - The sensor has a simple operation method. DETAILED DESCRIPTION - Ionic liquid functionalized graphene arc Vibrio deoxyribonucleic acid electrochemistry sensor is claimed. The electrochemistry sensor is prepared by Vibrio deoxyribonucleic acid electrochemistry sensor uses a gold electrode as a base electrode, an ionic liquid functionalized graphene-ZIF-8 composite membrane as an electrode modification material, and a probe single-stranded deoxyribonucleic acid immobilized on the surface of the modified electrode by covalent bonding of the bioligand 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and N-hydroxysuccinimide; and the ionic liquid functionalized graphene-ZIF-8 complex is prepared by first preparing ZIF-8 in a graphene oxide dispersion, and covalently modifying graphene-ZIF-8 complex with an amino functionalized ionic liquid to obtain ionic liquid functionalized graphene-ZIF-8 complex; and the preparation of (I), includes (i) preparing graphene oxide by Hummer method, and dispersing obtained graphene oxide in deionized water, and ultrasonically dispersing for 2 hours to obtain graphene oxide dispersion having a concentration of 0.5-1 mg/ml; and adding 10 ml of zinc nitrate hexahydrate methanol solution having a concentration of 90 mg/ml to above graphene oxide dispersion under stirring at 25-60 degrees C, adding 10 ml 2-methylimidazole methanol solution having a concentration of 900 mg/ml to the above mixture under the same conditions, stirring for 15 minutes at the same temperature 24 hours, centrifuging and collecting the grey white precipitate, washing for 3 times by water and methanol and naturally drying to obtain the GO-ZIF-8 complex, taking 40 mg of GO-ZIF-8 complex and dispersing it in deionized water to a concentration of 1 mg/ml, performing ultrasonic treatment for 30 minutes after slowly adding 20 mg IL and 20 mg potassium hydroxide, then performing ultrasonic treatment for 30 minutes, heating the ultrasonic dispersion and stirring at 50-100 degrees C for 24 hours, and centrifuging at a speed of 4000 revolutions/minute for 5 minutes, washing with deionized and anhydrous ethanol three times and drying to obtain IL-GR-ZIF-8 complex, (ii) polishing gold electrode with 0.05 mu m aluminum oxide polishing powder, washing with double distilled water, and ultrasonicating in an ultrasonic water bath for 5 minutes, and drying with high purity nitrogen gas; and ultrasonically dispersing IL-GR-ZIF-8 complex obtained in the step (i) in the deionized ion, formulating as a dispersion at a concentration of 3 mg/ml, applying 0.5-50 mu l of the dispersed droplets to the surface of the treated gold electrode, and allowing to dry naturally at room temperature top obtain gold electrode modified with IL-GR-ZIF-8 as IL-GR-ZIF-8/Au, (iii) immersing IL-GR-ZIF-8/Au in a mixed solution of 0.4 mol/l 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and 0.1 mol/l N-hydroxysuccinimide in 30 minutes, and the modified electrode surface carboxylation: taking 1-100 mu l probe single-stranded deoxyribonucleic acid solution at a concentration of 1x 10(-6) mol/l onto the surface of the modified electrode, and incubating at 25-70 degrees C for 2 hours, rinsing the electrode sequentially with phosphate-buffered saline having a pH of 7.4 and double distilled water, removing unfixed probe single-stranded deoxyribonucleic acid and drying naturally at room temperature to obtain deoxyribonucleic acid sensor. Au = gold electrode; GO = graphene oxide; GR = graphene; ionic liquid functionalized graphene-ZIF-8 = IL-GR-ZIF-8; and IL = ionic liquid (1-methyl-3-aminopropylimidazole tetrafluoroborate moiety of formula (I).