▎ 摘　　要

NOVELTY - A hybrid material comprises nano-flake graphene oxide-loaded chiral metal organic framework crystal composition containing copper complex (I) and having asymmetrical structure unit. USE - Chiral metal-organic frame material-graphene hybrid material is used for electrochemical sensing detection of enantiomer (claimed). DETAILED DESCRIPTION - A hybrid material comprises nano-flake graphene oxide-loaded chiral metal organic framework crystal composition containing copper complex of formula: (Cu(L-Asp)(4,4'-Bipy)2)n (I) and having asymmetrical structure unit. L-Asp2-=L-aspartate anion;and -Bipy=4,4'-bipyridyl. INDEPENDENT CLAIMS are included for the following: (i) preparation of chiral metal-organic frame material-graphene hybrid material, which involves blending alkaline aqueous solution of L-aspartic acid with aqueous solution of copper nitrate-graphene oxide, adding 4,4'-dipyridyl ethanol solution, maintaining the solution still for overnight, centrifuging and separating mixture, washing mixture for three times with ethanol and drying mixture; and # (ii)usage of chiral metal-organic frame material-graphene hybrid material for electrochemical sensing detection of enantiomer, which involves preparing chiral metal-organic frame material-graphene sensor working electrode in alumina powder which is polished, cleaning electrode with clean water and ethanol, diluting to 6 mu L chiral metal-organic frame-graphene solution, drying at room temperature to obtain chiral metal-organic frame-graphene hybrid electrode modified sensor working electrode, mixing chiral metal-organic frame-graphene hybrid material solution, 3 mg chiral metal-organic frame-graphene hybrid material, 250 mu L isopropanol, 720 mu L water and 30 mu L Nafion (RTM: sulfonated tetrafluoroethylene) blend and ultrasonically processing mixture in system for 10-15 minutes, preparing chiral metal-organic frame-graphene electrochemical chiral sensor, connecting working electrode, saturated calomel electrode as reference electrode and platinum wire electrode as counter electrode to electrochemical workstation, detecting enantiomers of R-2-amino-3-phenylpropionic acid and S-2-amino-3-phenylpropionic acid at pH of 7, adding 0.1 mol.L-1 phosphate-buffered saline buffer solution, carrying out differential pulse voltammetry using chiral metal-organic frame-graphene electrochemical chiral sensor, measuring current values of standard solutions of R-2-amino-3-phenylpropionic acid and S-2-amino-3-phenylpropionic acid at different concentrations and drawing working curve of enantiomers of R-2-amino-3-phenylpropionic acid and S-2-amino-3-phenylpropionic acid with respect to chiral metal-organic frame-graphene electrochemical chiral sensor.