▎ 摘　　要

NOVELTY - Method (M1) for altering electric impedance to an alternating electric field in a target site of a subject involves (a) introducing a nanoparticle to a target site in the subject, and (b) applying an alternating electric field to the target site of the subject, where the electric impedance in the target site of the subject to the alternating current is altered. USE - The method (M1) is useful for altering electric impedance to alternating electric field in target site of subject for treating tumor. The method (M4) is useful for reducing the viability of a cell. The target site is a tumor target site. The cell is a cancer or tumor cell. The cancer cells are glioblastoma cells, uterine sarcoma cells, breast adenocarcinoma cells, pancreatic cancer cells, non-small cell lung cancer, hepatocellular, gastric cancer cells, or brain cancer cells (all claimed). DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following: (1) a method (M2) for increasing efficacy of an alternating electric field in a target site of a subject involves (a) introducing a nanoparticle to a target site in the subject, and (b) applying an alternating electric field to the target site of the subject, where efficacy of the alternating electric field in the target site of the subject is increased; (2) a method (M3) for improving transport of a nanoparticle across a cell membrane of a cell involves (a) applying an alternating electric field to the cell for a period of time, where application of the alternating electric field increases permeability of the cell membrane, and (b) introducing the nanoparticle to the cell, where the increased permeability of the cell membrane enables the nanoparticle to cross the cell membrane; and (3) a method (M4) for reducing the viability of a cell involves (a) applying a first alternating electric field at a first frequency to the cell for a first period of time, where application of the first alternating electric field at the first frequency to the cell for the first period of time increases permeability of the cell membranes of the cell, (b) introducing a nanoparticle to the cell, where increased permeability of the cell membranes enables the nanoparticle to cross the cell membrane, and (c) applying a second alternating electric field at a second frequency to the cell for a second period of time, where the second frequency is different from the first frequency, and where the second alternating electric field at the second frequency reduces viability of the cell.