▎ 摘　　要

NOVELTY - A three-dimensional porous antibacterial silk fibroin/graphene/titanium dioxide bone tissue engineering scaffold preparing method involves dissolving a degummed silk in an aqueous solution of lithium bromide, removing impurities and lithium bromide to obtain an aqueous solution of regenerated silk fibroin and concentrating to obtain a solution A. A graphene oxide dispersion, the titanium dioxide hydrosol and the ethanol are blended. The graphene/titanium dioxide composite nanoparticles are synthesized by hydrothermal method, the powder is washed, dried, dispersed in water to obtain a mixed solution, added to the solution A and concentrated to form a regenerated silk fibroin/graphene/titanium dioxide mixed solution. An aqueous ethanol solution is added to the obtained mixed solution and allowed to stand to form a gel after mechanical stirring. The gel is placed in an ultra-low temperature, in a freeze dryer and vacuum dried to obtain the finished product. USE - Method for preparing three-dimensional porous antibacterial silk fibroin graphene titanium dioxide bone tissue engineering scaffold. ADVANTAGE - The method enables synergistically enhancing the regenerated silk fibroin material, improving the mechanical properties of the regenerated silk fibroin stent and imparting the antibacterial and osteoinductive functions of the stent. DETAILED DESCRIPTION - A three-dimensional porous antibacterial silk fibroin/graphene/titanium dioxide bone tissue engineering scaffold preparing method involves dissolving a degummed silk in an aqueous solution of lithium bromide, removing impurities and lithium bromide by centrifugation, filtration and dialysis to obtain an aqueous solution of regenerated silk fibroin and concentrating it to a mass percentage of regenerated silk fibroin of 1-4 %, which is recorded as solution A. A graphene oxide dispersion, the titanium dioxide hydrosol and the ethanol are blended, where the mass ratio of the graphene oxide to the titania is 1:1-1:10. After ultrasonically dispersing the mixed solution uniformly, the graphene/titanium dioxide composite nanoparticles are synthesized by hydrothermal method, the powder is washed and dried by suction filtration. The graphene/titanium dioxide powder is ultrasonically dispersed in water to obtain a mixed solution, which is added to the solution A, according to the graphene/titanium dioxide composite nanoparticle and the regenerated silk fibroin the protein mass ratio is 1:20-1:100, and the mixture is further concentrated to form a regenerated silk fibroin/graphene/titanium dioxide mixed solution with a percentage of regenerated silk fibroin of 4-15 %. An aqueous ethanol solution is added to the regenerated silk fibroin/graphene/titanium dioxide mixed solution and allowed to stand to form a gel after mechanical stirring. The gel is placed in an ultra-low temperature, in a freeze dryer and vacuum dried to obtain the three-dimensional porous antibacterial silk fibroin/graphene/titanium dioxide bone tissue engineering scaffold.