▎ 摘　　要

NOVELTY - Hydrogel material assembled from polyphenol and protein composite micro-nano particles, which is assembled and formed through hydrophobic interaction and hydrogen bond interaction between polyphenol/protein composite micro-nano particles, is claimed. After the hydrogel material is formed through hydrophobic interaction and hydrogen bond interaction between polyphenol/protein composite micro-nano particles, then adding an oxidizing agent to make the covalent crosslinking between polyphenols realize the crosslinking between polyphenol/protein composite micro-nanoparticles, then by adding a crosslinking agent, the protein chains are covalently crosslinked to form stable micro-nano particles. USE - The hydrogel material is used in preparing antiinflammation, anti-oxidation and antibacterial scaffolds for repairing and filling wounds or defects of bone tissue, cartilage tissue, muscle, blood vessel and skin, or preparing carriers or stents containing medicinal components, or preparing superficial skin and subcutaneous filler applications, preferably in the application of medical aesthetic fillers, or preparing biological ink, preferably the bioprinting ink loaded with living cell printing, or preparing tissue-adhesive gel materials, or slow-release carrier of metal ions to achieve wound repair. ADVANTAGE - The hydrogel material has good stability, and can be prepared without the need of organic reagent or surfactant used in the preparation process of traditional protein particles, but only uses the strong interaction between the poly phenol and protein to realize particle synthesis, greatly reduces the production cost of protein particles. The preparation method is suitable for different kinds of protein materials. Because the method does not use the use of surfactants or organic reagents, the method is simple and cost-efficient. The method can be used for preparing protein particles with universality. The protein chain can be covalently crosslinked to form stable micro-nano particles by adding crosslinking agent. The composite particle prepared by the method and the gel material has better stability. DETAILED DESCRIPTION - Hydrogel material assembled from polyphenol and protein composite micro-nano particles, which is assembled and formed through hydrophobic interaction and hydrogen bond interaction between polyphenol/protein composite micro-nano particles, is claimed. After the hydrogel material is formed through hydrophobic interaction and hydrogen bond interaction between polyphenol/protein composite micro-nano particles, then adding an oxidizing agent to make the covalent crosslinking between polyphenols realize the crosslinking between polyphenol/protein composite micro-nanoparticles, the polyphenol/protein composite micro-nanoparticles are formed by inducing assembly through hydrogen bond interaction, hydrophobic interaction or electrostatic interaction between protein polymer chains and polyphenols, preferably after the polyphenol/protein composite micro-nanoparticles are formed through hydrogen bond interaction, hydrophobic interaction or electrostatic interaction between protein polymer chains and polyphenols, then by adding a crosslinking agent, the protein chains are covalently crosslinked to form stable micro-nano particles. INDEPENDENT CLAIMS are also included for: hydrogel material assembled from micro-nano colloidal particles composited by polyphenols, proteins and metal ions, which is assembled and formed through hydrophobic interaction, hydrogen bond interaction and metal coordination interaction between polyphenol/protein composite micro-nano particles, preferably after the hydrogel material is formed through the hydrophobic interaction, hydrogen bonding and metal coordination between the polyphenol/protein composite micro-nanoparticles, then adding an oxidizing agent to make covalent crosslinking between polyphenols realize the crosslinking between polyphenol/protein composite micro-nano particles, the polyphenol/protein composite micro-nano particles are obtained by adding metal ions to the polyphenol/protein composite micro-nano particles to form coordination with polyphenols in the particles; hydrogel material assembled from rigid nanoparticle-polyphenol/protein composite core-shell structure micro-nanoparticles, which is assembled and formed through hydrophobic interaction and hydrogen bond interaction between core-shell structure micro-nano particles, preferably after the hydrogel material is formed through the hydrophobic interaction and hydrogen bond interaction between the composite micro-nano colloidal particles, then adding oxidizing agent to make the polyphenols covalently crosslink to realize the cross-linking between the core-shell structure micro-nanoparticles, the core layer of the core-shell micro-nanoparticle is composed of rigid nanoparticle material, the shell layer is composed of polyphenol/protein composite particles, the polyphenol/protein composite particles are formed through hydrogen bond interaction, hydrophobic interaction and electrostatic interaction between protein molecules and polyphenol molecules, preferably after the protein molecule and the polyphenol molecule are formed through hydrogen bond interaction, hydrophobic interaction and electrostatic interaction, the protein chain is covalently crosslinked by adding a crosslinking agent to form stable micro-nano particles; core-shell structure colloidal particle material composited by rigid nanoparticles-polyphenol/protein/metal ions, which is assembled and formed through hydrophobic interaction, hydrogen bond interaction and metal coordination interaction between core-shell structure micro-nano particles, preferably after the hydrogel material is formed through the hydrophobic interaction, hydrogen bonding and metal coordination between the core-shell structure micro-nanoparticles, then adding oxidizing agent to make the polyphenols covalently crosslink to realize the crosslinking between the core-shell structure micro-nanoparticles, the core layer of the micro-nanoparticles with core-shell structure is composed of rigid nanoparticle materials, the shell layer is composed of polyphenol/protein/metal composite particles, and the polyphenol/protein/metal composite particles are obtained by adding metal ions to the polyphenol/protein composite particles to form coordination with polyphenols in the particles; and preparing the hydrogel material.