▎ 摘　　要

NOVELTY - Single molecule gene sequencing comprises (i) growing quantum dot particles on the silicon carbide substrate, using a scanning electron microscope locating each quantum dot, , attaching graphene thin film, tagging deoxyribo nucelic acid molecule in a quantum dot thin film gap and epitaxial single-electron electrode array on the graphene film, (ii) using electromagnetic guidance and micro-fluidic technology, introducing a test deoxyribo nucelic acid molecule into graphene film and a silicon carbide substrate, continuously distorting electronic cloud near the quantum point structure. USE - The method is useful for single molecule gene sequencing (claimed). DETAILED DESCRIPTION - Single molecule gene sequencing comprises (i) growing quantum dot particles on the silicon carbide substrate, using a scanning electron microscope locating each quantum dot, , attaching graphene thin film, tagging deoxyribo nucelic acid molecule in a quantum dot thin film gap and epitaxial single-electron electrode array on the graphene film, (ii) using electromagnetic guidance and micro-fluidic technology, introducing a test deoxyribo nucelic acid molecule into graphene film and a silicon carbide substrate, continuously distorting electronic cloud near the quantum point structure, fluctuation of the electron cloud further affecting the electron distribution of graphene, electronic distribution for single electron graphene electrode array, through fast electronics output to computer, (iv) noting time speed signal output from single-electron electrode array to reconstruct deoxyribo nucelic acid molecule and a serpentine track information, (v) using the system response matrix of the known deoxyribo nucelic acid segment test electrode array, precision of the system response matrix sufficient to distinguish four nucleotides to the different electrodes, (vi) taking the current of single electron electrode near the track vector, output by inverting the system response matrix, calculating a sequence of vector composed of nucleotide species. An INDEPENDENT CLAIM is also included for monolayer gene sequencing device comprising a quantum dot film clearance module, a deoxyribo nucelic acid molecule movement control module, a single electronic reading module, a system response matrix calculation module and a deoxyribo nucelic acid sequence estimation module, where the quantum dot film clearance module, electronics, film for forming sequencing gap structure dynamics, mainly works in deoxyribo nucelic acid sequencing, the deoxyribo nucelic acid molecule movement control module is for controlling the deoxyribo nucelic acid molecule in the film gap The device precisely control the speed and acceleration of the DNA molecule, a single electronic reading module for single-electron device, the potential charge change real-time measurement, the graphene film is finely stored in digital form. The system response matrix calculating module for calculating each one pixel in thin gap of the single electron electrode array of the system response matrix, deoxyribo nucelic acid sequence estimation module is for estimating the deoxyribo nucelic acid sequence and mainly inverse one large-scale linear equation. DESCRIPTION OF DRAWING(S) - The diagram shows a schematic representation of monolayer gene sequencing device