▎ 摘　　要

NOVELTY - Biosensor comprises miR-205, hairpin nucleic acid probe HCR1, hairpin nucleic acid probe HCR2, graphene oxide and buffer. The nucleotide sequence of miR-205 comprises fully defined sequence of 22 nucleotides (SEQ ID NO: 1) as given in the specification, the nucleotide sequence of the hairpin nucleic acid probe HCR1 contains sequence A and sequence B. The nucleotide sequence of the hairpin nucleic acid probe HCR2 contains sequence Basterisk and sequence C, sequence-A is complementary to the nucleotide sequence of miR-205, sequence-B is complementary to sequence Basterisk, and sequence C is fully defined sequence of 22 nucleotides (SEQ ID NO: not defined) as given in the specification. The hairpin nucleic acid probes HCR1 and HCR2 have fluorescein attached to the 5'ends of the nucleotide sequences. USE - The biosensor is useful for detecting miR-205 in non-disease diagnosis by qualitative detection or quantitative detection (claimed). ADVANTAGE - The biosensor utilizes the biocompatibility and fluorescence quenching effect of graphene oxide through the design of hairpin nucleic acid probes HCR1 and HCR2, completes the detection of target miR205 in living cells and the quantitative detection and targeted detection of miR205 in the solution to be tested, detection of miR205 as a biomarker is more accurate, real-time and efficient. DETAILED DESCRIPTION - Biosensor comprises miR-205, hairpin nucleic acid probe HCR1, hairpin nucleic acid probe HCR2, graphene oxide and buffer. The nucleotide sequence of miR-205 is 5-uccuucauuccaccggagucug-3 (SEQ ID NO. 1), the nucleotide sequence of the hairpin nucleic acid probe HCR1 contains sequence A and sequence B. The nucleotide sequence of the hairpin nucleic acid probe HCR2 contains sequence Basterisk and sequence C, sequence-A is complementary to the nucleotide sequence of miR-205, sequence-B is complementary to sequence Basterisk, and sequence C is 5-tccttcattccaccggagtctg-3 (SEQ ID NO. not defined). The hairpin nucleic acid probes HCR1 and HCR2 have fluorescein attached to the 5'ends of the nucleotide sequences. An INDEPENDENT CLAIM is also included for method for non-disease diagnosis of miR-205 using the biosensor, comprising adding graphene oxide into the solution containing HCR1 and HCR2 and incubating to obtain a graphene oxide solution loaded with hairpin nucleic acid probes, co-culturing with cells, and performing laser confocal imaging.