▎ 摘　　要

NOVELTY - Preparation of gold nano cage/amino graphene based constructed avian herpes virus antigen sensor involves polishing glassy carbon electrode using 1.0, 0.3 and 0.05 mu m aluminum oxide polishing powder, cleaning with ultrapure water, placing electrode in 5 mmol/L potassium ferricyanide solution, scanning at - 0.2-0.6 V potential so that peak potential difference is less than 110 mV; placing in 2 mL 1% chloroauric acid and scanning at - 0.2 V for 30 seconds to get electrodeposited gold nanoparticles electrode; and adding 6 mu L 5-10 mu g/mL avian herpes virus antigen capture antibody Ab1 dropwise. USE - Method for preparing gold nano cage/amino graphene based constructed avian herpes virus antigen sensor (claimed). ADVANTAGE - The sensor has high specific surface area and excellent catalytic performance, and is biocompatible. The raw materials used improve sensitivity, stability and immunity of sensor. DETAILED DESCRIPTION - Preparation of gold nano cage/amino graphene based constructed avian herpes virus antigen sensor comprises polishing glassy carbon electrode using 1.0, 0.3 and 0.05 mu m aluminum oxide polishing powder, cleaning with ultrapure water, placing electrode in 5 mmol/L potassium ferricyanide solution, scanning at - 0.2-0.6 V potential so that peak potential difference is less than 110 mV; placing in 2 mL 1% chloroauric acid and scanning at - 0.2 V for 30 seconds to get electrodeposited gold nanoparticles electrode; adding 6 mu L 5-10 mu g/mL avian herpes virus antigen capture antibody Ab1 dropwise, drying at 4 degrees C and washing with ultrapure water; adding 3 mu L 0.2-1 wt.% bovine serum albumin solution to electrode surface, drying at 4 degrees C and washing with ultrapure water; adding 6 mu L series of different concentrations of avian herpes virus antigen solution to electrode surface, drying at 4 degrees C and washing with ultrapure water; and adding 4-6 mu L detection antibody incubated gold nano cage/amino graphene-Ab2 solution to electrode surface, incubating at 4 degrees C in refrigerator for 1 hour, cleaning and drying. An INDEPENDENT CLAIM is included for application of gold nano cage/amino graphene based constructed avian herpes virus antigen sensor comprising using electrochemical workstation with three electrode system for measuring, taking gold nano cage/amino graphene based constructed avian herpes virus antigen sensor as working electrode, saturated calomel electrode as counter electrode, platinum wire electrode as auxiliary electrode and phosphate buffer solution with 7.0 pH, scanning using time-current method with - 0.4 V for 400 seconds and recording current changes; recording test current changes in 10 mL phosphate buffer solution with 7.0 pH and 0.001-50 ng/mL avian herpesvirus antigen standard solution, where according to resulting current difference with avian herpesvirus antigens concentration drawing working curve is linear; and testing sample solution to be tested instead of avian herpes virus antigen standard solution.