▎ 摘　　要

NOVELTY - A capacitive immunosensor for the detection of Vibrio cholerae comprises anatase titanium dioxide (ant-TiO2) and reduced graphene oxide (RGO) nanohybrid conjugate fabricated on indium-tin-oxide (ITO) coated glass plates, where the anti-TiO2:RGO-ITO electrode is functionalized with protein conjugated anti-Vibrio cholerae antibodies (Ab-Vc) and bovine serum albumin (BSA). USE - For the detection of Vibrio cholerae (claimed) bacteria in e.g. water, food, blood, feces, and the like, contaminated with such bacteria. ADVANTAGE - The capacitive immunosensor is highly sensitive biosensor for Vibrio cholerae detection which is simpler, much quicker and more reliable than the other kinds of test available for detecting Vibrio cholerae. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following: (1) preparation of ant-TiO2:RGO/ITO electrode involving preparing well dispersed stock solution of RGO and TiO2 (1:1) in organic compound by ultrasonication at 50W and 0.25 A for about 3 hours; dispersing 100 mu L of each stock solution in 10 mL of acetonitrile to make the colloidal suspension of RGO and TiO2; electrophoretically depositing the ant-TiO2:RGO/ITO electrode using a two-electrode cell by applying DC voltage (100 V) for 2 minutes; adding 10-5-10-4 mol of Mg(NO3)2.6H2O to the suspension that act as electrolyte for the electrophoretic deposition; dipping the anode and cathode in the colloidal suspension of anti-TiO2:RGO/ITO; removing the ant-TiO2:RGO/ITO films from the suspension followed by washing with deionised water and drying; and (2) functionlization of ant-TiO2:RGO/ITO electrode involving loading 8-12 lambda of protein conjugated anti-Vibrio cholera solution onto the surface of ant-TiO2/RGO/ITO; risnsing the electrode with phosphate buffer to remove any unbound Ab-Vc; treating the Ab-Vc/ant-TiO2/RGO/ITO immunoelectrode with BSA (1mg/ml) solution for 4 hours to block non-specific sites.