▎ 摘　　要

NOVELTY - Fabrication of electrochemical biosensor platform for label-free evaluation of ligand-receptor interaction, involves washing a screen printed electrode with water; injecting a ligand onto working electrode of screen printed electrode surface; washing ligand injected screen printed electrode with ultrapure water for 2-3 times after 2-3 hours; adding a nanomaterial and leaving at room temperature for 2-3 hours for incubation; immobilizing a protein over the screen printed electrode surface by incubating for 20 minutes under ambient conditions; washing with either phosphate buffered saline (PBS) of 0.01 M at pH of 7.4 after 20 minutes or adding 10 mu l antibody and washing with the PBS after leaving overnight at 4 degrees C, to remove unbound protein; and adding blocking buffer if sample is not pure. The method of fabrication provides a detection platform that is suitable for different types of electrodes with a lower detection limit of 0.63 fm and detects up to molecular size of 200 daltons. USE - The method is useful for fabrication of electrochemical biosensor platform for label-free evaluation of ligand-receptor interaction (claimed). The biosensor is useful for screening drug molecules, protein-protein interaction affinity, protein-ligand (small molecules) affinity, and antibody-antigen or antibody-hormone interaction. ADVANTAGE - The method provide a highly sensitive, time dependent behavior and cost-effective electrochemical technique that can be suitable for both small as well as large ligand molecules.