▎ 摘　　要

NOVELTY - Enzyme immobilization promoter comprises polyethyleneimine functionalized graphene oxide and genipin in a mass ratio of 1-5:1. USE - The enzyme immobilization promoter is useful in functional modification of surface of conductive reaction zone of working electrode of three-electrode detection structure, which is useful for preparing glucose sensor, lactic acid sensor, glutamic acid sensor, uric acid sensor, ascorbic acid sensor or dopamine sensor, and implanted adenosine continuous detection sensor (all claimed) for real-time monitoring of acupuncture-induced local adenosine release in acupoints in vivo. ADVANTAGE - The flexible implantable adenosine sensor with a three-electrode structure has characteristics of good stability, high sensitivity, wide linear detection range, short response time, and strong anti-interference; can realize the in vivo continuous detection of changes in the concentration of adenosine in the animal body and brain, and provides a brand-new modern detection method for the in-depth study of the mechanism of acupuncture effect. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: Enzyme cross-linked fixing liquid, comprising a polyethyleneimine functionalized graphene oxide solution and multienzyme cascade reaction solution with a volume ratio of 1-3:1; Functional modification of surface of conductive reaction zone of the working electrode; and Three-electrode detection structure. DESCRIPTION OF DRAWING(S) - The drawing shows a perspective view of a three-electrode detection structure of an enzyme immobilization promoter. 1Silver foil 2Flexible film base material 4Insulating layer 5Conductive reaction zone