▎ 摘　　要

NOVELTY - Argonaute protein-based field effect transistor nucleic acid sensor comprises insulating substrate, ultra-thin semiconductor material layer arranged on an insulating substrate, and source and drain electrodes are set at both ends of the ultra-thin semiconductor material layer. The surface of the ultra-thin semiconductor material is modified with Argonaute protein and a guide chain. USE - Argonaute protein-based field effect transistor nucleic acid sensor. ADVANTAGE - The field effect transistor sensor is placed in a detection solution, an electrical test device is connected, and target nucleic acid is added into the solution during detection, so that detection of trace nucleic acid analytes is realized. The method compared with the traditional nucleic acid targeted optical test method used for detecting nucleic acid at present has the advantages of no need of amplification, greatly shortened detection time, high sensitivity, good specificity and good application prospect. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: (1) a method for preparing the Argonaute protein-based field effect transistor nucleic acid sensor, which involves: (a) processing a source drain electrode on an insulating substrate; (b) transferring the ultra-thin semiconductor material to an insulating substrate, etching the ultra-thin semiconductor material into a specific shape by utilizing a photo etching technology, and connecting the specific shape between a source electrode and a drain electrode for preparing a field effect transistor device with an ultra-thin semiconductor material channel exposed outside; (c) modifying connecting molecules on an ultra-thin semiconductor material channel of the prepared device; (d) modifying and fixing Argonaute protein and the guide chain on the surface of the ultra-thin semiconductor material channel; and (e) making liquid bath on the field effect transistor and then kept for future use; and (2) an application of the Argonaute protein-based field effect transistor nucleic acid sensor, which is used for detecting target nucleic acid, where target nucleic acid to be detected is added into liquid tank, so that target nucleic acid contacts with a semiconductor channel, and high-sensitivity detection of the target nucleic acid to be detected is realized through electric signal change, target nucleic acid at the time of the test is single-stranded DNA or RNA, and specific detection method of the target nucleic acid to be detected specifically involves connecting the source electrode of the field effect transistor device with the prepared ultra-thin semiconductor material channel exposed outside with the anode of an electrical test system, connecting the drain electrode with the cathode of the electrical test system, erecting liquid tank on the ultra-thin semiconductor material channel, and adding 10-100 microliters of reaction buffer solution into the liquid tank.