▎ 摘 要
NOVELTY - Preparing self-assembled nano sphere, comprises: immersing loading material into dispersion of graphene oxide for 10 minutes under ultrasonic condition, and then drying with nitrogen to form nanoparticles, and then immersing the nanoparticles into chitosan solution for 10 minutes, then drying with nitrogen, and then repeated the above steps to prepare multilayer nanospheres; and then immersing the multilayer nanospheres into 4,4'-diaminostilbene-2,2'-disulfonic acid solution (DAS) for 30 minutes and then taking the multilayer nanospheres out, drying with nitrogen, and irradiating by UV to prepare sustained-release self-assembled nanospheres. USE - The method is useful for preparing self-assembled nano sphere, where the self-assembled nano sphere is useful for controlling the sustained-release of small molecules (claimed). ADVANTAGE - The method: provides the nano sphere that has loading and sustained release to realize the selective release of small molecular loading material and the precise control of release rate and can be hydrated into a film during use; and utilizes the hydrophilic group of the chitosan making the self-assembled nanosphere that has film-forming property and biological adhesion property.