▎ 摘　　要

NOVELTY - A device providing electrical stimulation and shear force to cells placed in flat flow cavity micro-structure on bottom electrode plate is composed of: three layers including flat upper plate, and upper and lower layers of gasket; where upper plate has fluid inlet and outlet, upper gasket has flow guide groove and rectangular slit plate, lower gasket has cubic flow chamber (18) having electrode plate (16), and comprises sheet having micro-structure having polydimethylsiloxane (PDMS) film and PDMS film with graphite film spin coated to it, and PDMS and graphite films have leads (8, 25). USE - As device capable of providing electrical stimulation and shear force to cells placed in flat flow cavity micro-structure on bottom electrode plate (claimed) which is useful for constructing tissues and organs useful for tissue engineering to maintain, repair, regenerate or improve damaged tissue and organ function. ADVANTAGE - The device integrates micro-structure, electrical frame stimulation and shearing force, which are capable of controlling cell adhesion, proliferation and differentiation, particularly to achieve synergistic effect; to improve biological function of cells, promote interaction of seed cells and encapsulating material, and promoting secretion of extracellular matrix from cells; whereas current flowing cavity device can not realize micro-structure along with electrical stimulation and shear force in single device. The device is capable of observing and researching directional movement of cells to several factors at the same time to stimulate response. The micro-topology structure of micro-structure substrate determines corresponding biological characteristics of cells. The flat electrode plate can realize uniform fluid shearing force. The shearing force can be changed by changing flat electrode plate. The device has simple, compact, and reliable structure of flat flow cavity micro-structure. Thus device is good for observing cell research with respect to combination of several factors to achieve combined stimulation of response, so as to realize accelerated tissue repair speed and improved tissue repair quality. By using the device it is easy to simulate in vivo cell micro-environment, thus can produce larger cell body in micro-environment; whereas current devices can deal with only one or two kinds of factors stimulation. In device each stimulating factor can be carried out in more than one mode and can be varied in strength; such as shearing force and electrode stimulation mode and intensity can be adjusted by using several electrodes at same time connected to peristaltic pump control, to realize multi-directional electrical stimulation signal generation. Also device comprises flat flow cavity capable of selecting different micro-topological structures according to requirements, and at the same time applies fluid shearing force stimulation at different modes and intensities. The graphene thin film used for micro-structure is electrically conductive, and at the same time does not cause toxicity to cells. DETAILED DESCRIPTION - A device capable of providing electrical stimulation and shear force to cells placed in flat flow cavity micro-structure on bottom electrode plate is composed of: three layers including flat upper plate, layer of upper gasket, and layer of lower gasket; where upper part plate is provided with fluid inlet and outlet, upper gasket is provided with flow guide groove and rectangular slit plate, lower gasket is provided with flow chamber (18), where flow chamber is cubic and is provided with electrode plate (16), where electrode plate comprises sheet having micro-structure, polydimethylsiloxane film (PDMS) and graphite film, PDMS film is fixed on thin slice having different micro-structures, graphene thin film is formed by spin-coating PDMS film on surface of electrode plate, and PDMS film and graphene thin film are respectively provided with leads (8, 25). The sheet having micro-structure is micro-groove array having depth of 100 nm to 20 mu m and 100 nm to 100 mu m, with distance between micro-grooves of 100 nm to 20 microns. The sheet having micro-structure has shape selected from triangle, quadrangle, pentagon or hexagon micro-dummy club matrix, where micro-dummy club each has side length of 100 nm to 20 microns, height of 100 nm to 100 microns, the distance between micro-dummy clubs is 100 nm to 20 mu m. The electrode plate is flat plate that shows uniform change in thickness, and is capable of holding sheet and PDMS film at 5-60 degrees wedge shaped inclination. The lead(s) is/are at the same time connected with one pole of power supply, and electrode plate is electrically connected with pair of electrodes (14, 17); or leads are respectively connected with two poles of power supply, electrode plate forms one of the electrodes, and current passes through electrode plate of cells to generate electrical stimulation. DESCRIPTION OF DRAWING(S) - The figure shows schematic diagram of the device capable of providing electrical stimulation and shear force to cells placed in flat flow cavity micro-structure on bottom electrode plate. Leads (8, 25) Electrodes (14, 17) Electrode plate (16) Flow chamber (18)