• 专利标题:   Detecting fresh taste amino acid based on nitrogen-doped graphene quantum dot fluorescent probe comprises e.g. preparing nitrogen-doped graphene quantum dot, taking hydrated citric acid and urea in de-ionized water to obtain mixed solution.
  • 专利号:   CN112067591-A
  • 发明人:   SUN C, WANG D, TAKADA T, YAN Z, XU W, ZHANG M, LI P
  • 专利权人:   JIANGSU AGRIC SCI INST
  • 国际专利分类:   G01N021/64
  • 专利详细信息:   CN112067591-A 11 Dec 2020 G01N-021/64 202004 Pages: 14 Chinese
  • 申请详细信息:   CN112067591-A CN10962954 14 Sep 2020
  • 优先权号:   CN10962954

▎ 摘  要

NOVELTY - Detection of fresh taste amino acid based on nitrogen-doped graphene quantum dot fluorescent probe comprises preparing nitrogen-doped graphene quantum dot, taking hydrated citric acid and urea in deionized water at ratio of 7:6 to obtain 10-50 mg/mL mixed solution, placing mixed solution in reaction kettle, hydrothermally reacting at 160 degrees C for 4 hours, cooling, dialyzing and purifying, freezing and drying to obtain product; constructing nitrogen-doped graphene quantum dot fluorescent probe, dissolving nitrogen-doped graphene quantum dot powder in water to obtain 100 mu g/mL nitrogen-doped graphene quantum dot solution; and measuring content of fresh taste amino acid, mixing soup pot sample to be tested with 100 mu g/mL nitrogen-doped graphene quantum dot solution in form of aqueous solution, measuring fluorescence emission spectrum to read fluorescence value, where excitation wavelength is 355 nm. USE - The method is useful for detecting fresh taste amino acid based on nitrogen-doped graphene quantum dot fluorescence probe. ADVANTAGE - The method enables detecting the fresh taste amino acid with better accuracy in a simple and rapid manner. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for nitrogen-doped graphene quantum dot fluorescent probe for detecting fresh amino acid.