▎ 摘　　要

NOVELTY - Method for producing a nucleic acid detection device involves (a) (i) printing a hydrophobic material to be spaced apart by a predetermined distance on a substrate having a hydrophilic surface in a roll-to-roll process, (ii) deploying the nanoparticles at air/liquid interface to form a nanoparticle monolayer, and (iii) performing a roll-to-roll LB (Langmuir-Blodgett) process, and laminating the nanoparticle single layer in a single layer or multi-layer in the reaction space on the substrate, or (b) printing a hydrophilic material to be spaced apart by a predetermined distance on a substrate having a hydrophobic surface in a roll-to-roll process, deploying the nanoparticles at the air/liquid interface to form a nanoparticle monolayer, and performing a roll-to-roll LB (Langmuir-Blodgett) process, and laminating the nanoparticle single layer in a single layer or multi-layer in the reaction space on the substrate. USE - The method is useful for producing nucleic acid detection device (claimed) to detect target nucleic acids such as DNA, and RNA present in a sample. ADVANTAGE - The method mass-produces nucleic acid detection device in which fluorescence signal is amplified more efficiently in a cost-effective manner within a short period of time. DETAILED DESCRIPTION - Method for producing a nucleic acid detection device involves (a) (i) printing a hydrophobic material to be spaced apart by a predetermined distance on a substrate having a hydrophilic surface in a roll-to-roll process, (ii) deploying the nanoparticles at air/liquid interface to form a nanoparticle monolayer, and (iii) performing a roll-to-roll Langmuir-Blodgett (LB) process, and laminating the nanoparticle single layer in a single layer or multi-layer in the reaction space on the substrate, or (b) printing a hydrophilic material to be spaced apart by a predetermined distance on a substrate having a hydrophobic surface in a roll-to-roll process, deploying the nanoparticles at the air/liquid interface to form a nanoparticle monolayer, and performing a roll-to-roll Langmuir-Blodgett (LB) process, and laminating the nanoparticle single layer in a single layer or multi-layer in the reaction space on the substrate, where a reaction space in form of a microwell in which a nucleic acid amplification reaction occurs is provided in the space between printed hydrophobic material and hydrophobic material. An INDEPENDENT CLAIM is included for nucleic acid detection device produced by the method.