▎ 摘　　要

NOVELTY - Preparing silk-spider silk composite silk fibroin nano-microsphere involves taking the cleaned spider silk, boiling in a sodium carbonate solution to degumming, and washing with water to obtain degummed spider silk. A purer silk fibroin solution is obtained. The spider silk protein solution is mixed and freeze-dried after low temperature storage to obtain a silk-spider silk composite silk fibroin solution. The silk-spider silk composite silk fibroin solution is putted into water, dissolved and placed in a phosphate buffer. A protease solution is added and heated the obtained mixed solution in a water bath. The protease solution is heated up, inactivated, cooled to room temperature and left to stand, freeze-dried to obtain silk-spider silk composite silk fibroin microspheres. The obtained mixed solution is centrifuged. The lower sediment is removed followed by freeze-drying to obtain silk-spider silk composite silk fibroin nano-microspheres containing chitosan-modified graphene oxide. USE - Method for preparing silk-spider silk composite silk fibroin nano-microspheres containing chitosan modified graphene oxide. ADVANTAGE - The method has good biological compatibility and biological degradability, promotes the release of the load medicine to sterilize, strength and elasticity and healing of the wound, is slowly release graphene oxide to inhibit wound bacterial growth, utilizes as wound dressing, effectively treats burn and scald wound, and has simple operation process, non-toxic and harmless, green and environment-friendly. DETAILED DESCRIPTION - Preparing silk-spider silk composite silk fibroin nano-microsphere involves taking the cleaned spider silk, boiling in a sodium carbonate solution to degumming, and washing with water to obtain degummed spider silk. The degummed spider silk is putted in an acetic acid solution for heating reaction. The obtained mixed solution is centrifuged after the reaction. The supernatant is collected to obtain a spider silk protein mixed solution, which is dialyzed in water to obtain a purer spider silk protein solution. The silk is taken, washed, putted in sodium carbonate solution to boil for degumming and washed with water to obtain degummed silk. The degummed silk is dissolved in a lithium bromide solution to obtain a silk fibroin mixed solution after dialysis in water. A purer silk fibroin solution is obtained. The spider silk protein solution is mixed and freeze-dried after low temperature storage to obtain a silk-spider silk composite silk fibroin solution. The silk-spider silk composite silk fibroin solution is putted into water, dissolved and placed in a phosphate buffer. A protease solution is added and heated the obtained mixed solution in a water bath. The protease solution is heated up, inactivated, cooled to room temperature and left to stand, freeze-dried to obtain silk-spider silk composite silk fibroin microspheres. The graphene oxide solution is mixed with the chitosan solution, the cross-linking agent and the catalyst. The reaction is stirred to make the graphene oxide and the chitosan covalently combine to obtain the graphene oxide hydrogel modified by the chitosan after cryopreservation and freeze-dried to obtain chitosan-modified graphene oxide particles. The chitosan-modified graphene oxide particles and the silk-spider silk composite silk fibroin microspheres are dispersed in water to form a suspension, ultrasonically mixed evenly. The obtained mixed solution is centrifuged. The lower sediment is removed followed by freeze-drying to obtain silk-spider silk composite silk fibroin nano-microspheres containing chitosan-modified graphene oxide.