▎ 摘　　要

NOVELTY - Preparing electro-chemiluminescence immunosensor involves dissolving zinc nitrate hexahydrate, polyvinylpyrrolidone, ruthenium (4,4'-dicarboxylic acid-2,2'-bipyridine) (Ru(dcbpy)32+) and pyrazine in water and reacting to obtain ruthenium-based metal organic framework (Ru-MOF) solution. The ammonium heptamolybdate ((NH4)6Mo7O24) is dissolved in the graphene oxide dispersion, maintained at 40-60degrees Celsius for 4-6 hours, thiourea is added, and heated to obtain a molybdenum disulfide doped with graphene oxide (MoS2-GO) solution. USE - Method for preparing electro-chemiluminescence immunosensor used for 5-FU detection (claimed) used in the chemotherapy technology. ADVANTAGE - The method enables to utilize the specific immunoreaction between the monoclonal antibody of the 5-fluorouracil and the antigen developed cooperatively to quantitatively detect the 5-fluorouracil, which improves the detection on the target, and selectivity of the object to be detected. The immunosensor uses simple instruments and device, is easy and convenient to operate, high in detection efficiency, simple in sample treatment, high in sensitivity and wide in linear range, convenient to popularize, low in cost and high in practical value. DETAILED DESCRIPTION - Preparing electro-chemiluminescence immunosensor involves dissolving zinc nitrate hexahydrate, polyvinylpyrrolidone, ruthenium (4,4'-dicarboxylic acid-2,2'-bipyridine) (Ru(dcbpy)32+) and pyrazine in water and reacting to obtain ruthenium-based metal organic framework (Ru-MOF) solution. The ammonium heptamolybdate ((NH4)6Mo7O24) is dissolved in the graphene oxide dispersion, maintained at 40-60degrees Celsius for 4-6 hours, thiourea is added, and heated to obtain a molybdenum disulfide doped with graphene oxide (MoS2-GO) solution. The MoS2-GO activation solution and Ru-MOF activation solution are obtained by activating the MoS2-GO solution and the Ru-MOF solution, respectively. The 5-fluorouracil (5-FU) antibody solution is added to the Ru-MOF activation solution, and reacted to obtain ruthenium-based metal organic framework/antibody (Ru-MOF/Ab) probe. The MoS2-GO activation solution is dropped on the surface of the glassy carbon electrode, and the 5-FU coated antigen solution is added dropwise to incubate after drying to obtain a ruthenium-based metal organic framework molybdenum disulfide doped with graphene oxide/ovalbumin (MoS2-GO/OVA) substrate, The Ru-MOF/Ab probe is dropped on the MoS2-GO/OVA substrate and reacted to obtain the electro-chemiluminescence immunosensor. An INDEPENDENT CLAIM is included for a method for using electro-chemiluminescence immunosensor in 5-FU detection, which involves: (a) using the electro-chemiluminescence immunosensor as the working electrode in the three-electrode system, performing cyclic voltammetry on the 5-FU standard sample, drawing luminescence intensity-concentration curve, and obtaining linear regression equation is obtained; and (b) performing electro-chemiluminescence (ECL) detection on the treated sample to obtain luminescence intensity, calculating luminescence intensity using the linear regression equation to obtain concentration of pentafluorouracil in the sample.