▎ 摘　　要

NOVELTY - Simulating effect of toxins on neural stem cells through blood-brain barrier involves adding primary mouse brain microvascular endothelial cells into endothelial cell culture tank filled with endothelial cell culture solution for culture, and enabling mouse brain microvascular endothelial cells to form single mouse brain microvascular endothelial cell layer at the bottom of endothelial cell culture tank. USE - Method for simulating effect of toxins on neural stem cells through blood-brain barrier for use in the study of simulating the early developmental abnormalities of nervous system caused by exposure to PM2.5 (claimed). ADVANTAGE - The method for simulating the effect of toxins passing through the blood-brain barrier on neural stem cells enables to provide theoretical reference and support for the prevention and treatment of neurodevelopment-related diseases, and brings about the research field of PM2.5 exposure-induced related neurodevelopment-related diseases to generate great promotion. DETAILED DESCRIPTION - Simulating effect of toxins on neural stem cells through blood-brain barrier involves adding primary mouse brain micro vascular endothelial cells into endothelial cell culture tank filled with endothelial cell culture solution for culture, and enabling mouse brain micro vascular endothelial cells to form single mouse brain micro vascular endothelial cell layer at the bottom of endothelial cell culture tank. The primary neural stem cells are added into a neural stem cell culture tank which is internally provided with graphene oxide porous cell bracket, and is filled with neural stem cell culture solution for culture. The neural stem cell culture tank is communicated with bottom of endothelial cell culture tank through micro-channel, toxin is added into endothelial cell culture tank, flow of liquid flowing into neural stem cell culture tank from endothelial cell culture tank is controlled through micro-channel, and physiological activity of neural stem cell is observed during culture process of neural stem cell. An INDEPENDENT CLAIM is included for a device for simulating toxins passing through the blood-brain barrier to act on neural stem cells, which comprises polydimethylsiloxane carrier for simulating a microenvironment in vivo, where polydimethylsiloxane carrier comprises an upper carrier and lower carrier which are bonded to each other, upper carrier is arranged on the upper surface of lower carrier and is arranged in a step shape with the lower carrier, upper carrier is provided with endothelial cell culture tank extending toward the lower carrier, first microchannel is horizontally connected with endothelial cell culture tank, and downwardly extending trunk is arranged at the bottom of endothelial cell culture tank microchannel; where bottom of endothelial cell culture tank is used for laying a layer of mouse brain microvascular endothelial active cells, and endothelial cell culture tank is used for adding vascular endothelial cell culture medium, two or more neural stem cell culture tanks are arranged on the stepped surface of carrier, graphene oxide porous cell bracket is fixed at the bottom of the neural stem cell culture tank, and neural stem cell culture tank is communicated with the trunk microchannel, where graphene oxide porous cell bracket is used to load the neural stem cells to be tested, first microchannel is also connected with a toxin addition port, and toxin to be tested enters endothelial cell culture tank through the toxin addition port, and passes through the mouse brain microvascular endothelial active cell layer, and trunk microchannel and first microchannel enter the neural stem cell culture tank.