• 专利标题:   Immune-detecting homogeneous phase virus, involves preparing conjugates, mixing quantum dot conjugates with different colors and adding excessive graphene oxide and viruses, and performing qualitative identification on viruses.
  • 专利号:   CN102608090-A, CN102608090-B
  • 发明人:   CHEN L, HE Z
  • 专利权人:   UNIV WUHAN
  • 国际专利分类:   G01N021/64
  • 专利详细信息:   CN102608090-A 25 Jul 2012 G01N-021/64 201351 Pages: 13 Chinese
  • 申请详细信息:   CN102608090-A CN10073965 20 Mar 2012
  • 优先权号:   CN10073965

▎ 摘  要

NOVELTY - Method for immune-detecting homogeneous phase virus, involves mixing quantum dot solution modified by streptavidin with different colors with biotinylation antibodies of different types of viruses to prepare conjugates, mixing the quantum dot conjugates with different colors and adding excessive graphene oxide to enable fluorescence of the quantum dot to be quenched, then adding viruses, performing qualitative identification on the types of the viruses and virus content, and performing half-quantitative detection on the viruses with respect to color variation. USE - The method is useful for immune-detecting homogeneous phase virus (claimed). DETAILED DESCRIPTION - Method for immune-detecting homogeneous phase virus, involves mixing a certain amount of quantum dot solution modified by streptavidin with different colors with biotinylation antibodies of different types of viruses to prepare conjugates, mixing the quantum dot conjugates with the different colors and then adding excessive graphene oxide to enable fluorescence of the quantum dot to be quenched, then adding viruses with respect to the antibodies to enable the fluorescence intensity of the quantum dot to be respectively rising, performing qualitative identification on the types of the viruses with respect to whether the fluorescence intensity of the quantum dot is rising or not, performing quantitative detection on virus content with respect to an rising intensity value of the fluorescence intensity of the quantum dot, or shooting by using an UV lamp to excite a sample solution in an ultraviolet analyzer to obtain a visible picture, and performing half-quantitative detection on the viruses with respect to color variation.