▎ 摘　　要

NOVELTY - Electrochemical detection of microcystins-LR by graphene signal amplification comprises adding 6-mercaptohexanol/gold nanoparticle/gold (MCH/AuNPs/Au) electrode with nucleic acid restriction enzyme reaction buffer solution, graphene-aptamer complex, microcystins-LR solution and nucleic acid enzyme solution, incubating MCH/AuNPs/Au electrode at 25-35 degrees C for 1-2 hours, washing using ethanol and ultra-pure water, removing weak combination of graphene, drying under nitrogen protection, taking out, electrochemical detecting, and measuring concentration of microcystins-LR solution. USE - Method for electrochemical detecting microcystins-LR by graphene signal amplification (claimed). ADVANTAGE - The method is simple and feasible, has portable cheap apparatus, quickly obtains result, realizes ultra-sensitive trace of microcystin-LR in environment, and uses electrochemical analysis technique and high-sensitivity nucleic acid aptamer combining property identification ability. DETAILED DESCRIPTION - Electrochemical detection of microcystins-LR by graphene signal amplification comprises adding 6-mercaptohexanol/gold nanoparticle/gold (MCH/AuNPs/Au) electrode with nucleic acid restriction enzyme reaction buffer solution, graphene-aptamer complex, microcystins-LR solution and nucleic acid enzyme solution at volume ratio of 9-11:4-6:2-4:1-3, incubating MCH/AuNPs/Au electrode at 25-35 degrees C for 1-2 hours, washing using ethanol and ultra-pure water, removing weak combination of graphene, drying under nitrogen protection, taking out, electrochemical detecting, and measuring concentration of microcystins-LR solution.