▎ 摘　　要

NOVELTY - A copper oxide/reduced graphene oxide adopt poly(N-isopropylacrylamide) composite self-supporting electrode preparing method involves mixing and dispersing porous copper oxide and graphene oxide in a uniform suspension and preparing a copper oxide/reduced graphene oxide composite membrane by suction filtration, where the mass ratio of the porous copper oxide to graphene oxide is 5:1-5:3 and the composite film has a thickness of 10-30 mu m, placing copper oxide/reduced graphene oxide composite membrane in an aqueous solution of poly(N-isopropylacrylamide) monomer and inserting into an ultrasonic, using continuous ultrasonic polymerization method and the polymerization reaction is carried out by intermittent nitrogen cycle, performing cumulative ultrasonic irradiation for 1 hour to obtain the finished product, the concentration of the poly(N-isopropylacrylamide) monomer aqueous solution is 10-30 mg/mL. USE - Method for preparing copper oxide/reduced graphene oxide adopt poly(N-isopropylacrylamide) composite self-supporting electrode for temperature-sensitive enzyme-free glucose sensor that is utilized for measuring glucose catalytic behavior. ADVANTAGE - The method enables preparing copper oxide/reduced graphene oxide adopt poly(N-isopropylacrylamide) composite self-supporting electrode with high complex degree, moderate and low energy consumption. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for the following: (1) a copper oxide/reduced graphene oxide adopt poly(N-isopropylacrylamide) composite self-supporting electrode is used as a working electrode, the saturated calomel electrode is a reference electrode and the platinum electrode is used as an auxiliary electrode to form a three-electrode temperature-sensitive glucose electrochemical sensor (2) a method for measuring a glucose catalytic behavior using the temperature-sensitive enzyme-free glucose sensor, involves placing three-electrode system in a weighing bottle containing a glucose and sodium hydroxide solution and placing in its entirety, measuring the catalytic behavior of glucose in different temperature water baths, when the test temperature is 25-30 degrees C, the sensor showed an "on" state, indicating that the poly(N-isopropylacrylamide) chain is not collapsed, when the test temperature is 35-45 degrees C, the sensor showed "off" "State, indicating that the poly(N-isopropylacrylamide) chain is collapsed.