▎ 摘　　要

NOVELTY - Preparing gold-graphene quantum dot-platinum-palladium based immunosensor comprises e.g. polishing glassy carbon electrode having diameter of 3-5 mm with aluminum oxide polishing powder, cleaning using ultra-pure water, carrying out electrodepositing on electrode surface using 1 wt.% chloroauric acid using chronoamperometry, drying, rinsing with ultrapure water, drying, coating 6 mu l tumor marker anti-Ab1 solution on electrode surface, placing in a refrigerator, drying at 4 degrees C, washing unbound capture antibody Ab1 with ultrapure water, coating bovine serum albumin (BSA) solution. USE - The method is useful for preparing gold-graphene quantum dot-platinum-palladium based immunosensor (claimed). ADVANTAGE - The gold-graphene quantum dot-platinum-palladium based immunosensor has strong specificity, high sensitivity and low detection limit. DETAILED DESCRIPTION - Preparing gold-graphene quantum dot-platinum-palladium based immunosensor comprises (i) polishing glassy carbon electrode having diameter of 3-5 mm with aluminum oxide polishing powder, cleaning using ultra-pure water; (ii) carrying out electrodepositing on electrode surface using 1 wt.% chloroauric acid at a voltage of -0.2-0.2 V using chronoamperometry, drying, rinsing with ultrapure water, drying; (iii) coating 6 mu l tumor marker anti-Ab1 solution (8-12 ~ mg/ml) on electrode surface, placing in a refrigerator, drying at 4 degrees C; (iv)washing unbound capture antibody Ab1 with ultrapure water, COATING 3 mu l bovine serum albumin (BSA) solution (0.5-1.5 mg/ml) on electrode surface, drying in a refrigerator at 4 degrees C; (iv) rinsing unbound BSA with ultrapure water, dropwise adding 6 mu l different concentrations carcinoembryonic antigen solution (1x 10-6-10 ng/ml), incubating at room temperature for 1 hour, cleaning using ultra-pure water cleaning, and drying by placing in a refrigerator at 4 degrees C; (vi) applying 6 mu l detected antibody gold-graphene quantum dot-platinum-palladium-antibody (Ab2) solution (1.5-5 mg/ml) to electrode surface in the incubator, incubating at room temperature for 1 hours, cleaning using ultra-pure water clean, drying by placing in a refrigerator at 4 degrees C, to obtain gold-graphene quantum dot-platinum-palladium based carcinoembryonic antigen sensor; (iv) washing unbound capture antibody (Ab1) with ultrapure water, adding 3 mu l bovine serum albumin (BSA) solution (0.5-1.5 mg/ml) on electrode surface, drying in a refrigerator at 4 degrees C; (iv) rinsing unbound BSA with ultrapure water, adding dropwise 6 ~ ml different concentrations of carcinoembryonic antigen solution (1x 10-6-10 ng/ml), incubating at room temperature for 1 hour, cleaning in ultra-pure water, drying by placing in a refrigerator at 4 degrees C; and (vi) adding 6 mu l gold-graphene quantum dot-platinum-palladium antibody (Ab2) solution in an incubator to electrode surface, incubating at room temperature for 1 hour, cleaning using ultra-pure water, drying by placing in a refrigerator at 4 degrees C, to obtain the final product. An INDEPENDENT CLAIM is also included for use of gold-graphene quantum dot-platinum-palladium based immunosensor in detection of carcinoembryonic antigen comprising (A) taking three-electrode system with an electrochemical workstation, saturated calomel electrode as reference electrode, platinum wire electrodes as auxiliary electrode, and prepared sensor as working electrode, adding 10 ml phosphate buffer solution (50 mmol/l) having pH 5.91-8.04, testing; (B) detecting carcinoembryonic antigen using chronoamperometry having input voltage is -0.4 V, sampling interval of 0.1 seconds, running time of 400 seconds; (C) providing stable background current, adding 10 ml phosphate buffer (50 mmol/l) every 50 seconds having pH of 7.4, adding 10 mu l hydrogen peroxide solution (5 mol/l), and recording the current change.