▎ 摘　　要

NOVELTY - Electrochemical sensor, comprises a coating that surrounds more than one electrodes and more than one enzymes distributed within coating. The more than one enzymes comprises peroxidase, and/or glucose oxidase. USE - The electrochemical sensor is useful for measurement of glucose concentration with more than one electrodes. ADVANTAGE - The sensor can measure the amount of sugar in food and drink, monitors blood glucose for diabetic people on regular basis to ensure healthy life. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for: (I) microfluid device, comprising electrochemical sensor; (II) electrochemical detection of glucose in sample, comprising exposing a fluid sample obtained from a subject to the electrochemical sensor, and detecting current generated from the oxidation of hydrogen peroxide (H2O2) during exposing, where current corresponds to the concentration of glucose in the fluid sample; (III) treating subject afflicted with or at risk of diabetes, comprising exposing a fluid sample obtained from a subject to the electrochemical sensor, detecting the current generated from the oxidation of H2O2 during exposing, where current corresponds to the concentration of glucose in the fluid sample, and treating the subject for diabetes if the subject has a fasting blood glucose concentration is greater than 100 mg/dl; (IV) diagnosing subject with or at risk of diabetes, comprising exposing a fluid sample obtained from a subject to the electrochemical sensor, detecting current generated from the oxidation of H2O2 during exposing, where current corresponds to the concentration of glucose in the fluid sample, and diagnosing the subject as having or at risk of having diabetes if the subject has a fasting blood glucose concentration is greater than 100 mg/dl; (V) monitoring subject having or at risk of having diabetes, comprising exposing a fluid sample obtained from a subject to the electrochemical sensor, detecting current generated from the oxidation of H2O2 during exposing, where current correlates to the concentration of glucose in the fluid sample, and monitoring the subject having or at risk of having diabetes; (VI) kit, comprising electrochemical sensor; and (VII) electrochemical sensor for the measurement of glucose concentration comprising more than one electrodes, a coating that surrounds the more than one electrodes, and more than one enzymes distributed within the coating, where more than one enzymes comprises manganese peroxidase and glucose oxidase immobilized onto more than one of the electrodes by polymerizing aniline, and optionally, where Nafion(RTM: Sulfonated tetrafluoroethylene) polymer is deposited on the coated more than one electrodes, or electrochemical sensor for measurement of glucose concentration, comprising more than one electrodes, a coating that surrounds the more than one electrodes, and more than one enzymes distributed within the coating, where more than one enzymes comprises manganese peroxidase and glucose oxidase immobilized onto more than one of the electrodes by polymerizing aniline, and, optionally, where more than one electrodes comprise a gold nanoparticle layer between the electrode and the coating.