• 专利标题:   Kit useful for detecting canine C-reactive protein, comprises DNA1-C-reactive protein (cCRP) antigen conjugate, DNA2-cCRP antibody conjugate, and labeled acridinium ester (AE) DNA3 and graphene oxide (GO) combined with antioxidant.
  • 专利号:   CN110836976-A
  • 发明人:   CAO D
  • 专利权人:   NANJING PUGUANG BIOTECHNOLOGY CO LTD
  • 国际专利分类:   G01N021/76, G01N033/68
  • 专利详细信息:   CN110836976-A 25 Feb 2020 G01N-033/68 202022 Pages: 8 Chinese
  • 申请详细信息:   CN110836976-A CN11274800 12 Dec 2019
  • 优先权号:   CN11274800

▎ 摘  要

NOVELTY - Kit for detecting canine C-reactive protein based on homogeneous chemiluminescence immunocompetitive method, comprises DNA1-C-reactive protein (cCRP) antigen conjugate, DNA2-cCRP antibody conjugate, and labeled acridinium ester (AE) DNA3 and graphene oxide (GO) combined with antioxidant (AOD). USE - The kit is useful for detecting canine C-reactive protein (claimed). ADVANTAGE - The kit detecting canine C-reactive protein simply and accurately. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for method for detecting canine C-reactive protein using the kit, which involves mixing calibration solution of different concentration or containing cCRP blood sample and the test solution in the kit in a volume ratio of 1:10, placing the HSCL-10000 chemiluminescence meter, incubating at 37 degrees C for 5 minutes, after incubation, adding 200 mu l chemiluminescent substrate through HSCL-10000 chemiluminescence meter, immediately passing the photomultiplier tube (PMT), detecting the chemiluminescence signal of solution, where the detection time is 3 seconds, measuring the luminescence value (RLU), and obtaining calibration curve of cCRP and the concentration of cCRP in the test sample.