▎ 摘　　要

NOVELTY - Preparing sulfosuccinimidyl-2-(biotinamido)ethyl-1,3-dithiopropionate (sulfo-NHS-biotin) modified indium nitride paste electrode sensor involves adding 48-52 wt.% nano indium nitride, 20-24 wt.% graphene oxide, 12-14 wt.% 1-aminopropyl-3-methylimidazole bromide, 6-8 wt.% mineral oil and 6-10 wt.% pentanol in agate mortar. The total mass percentage of each component is 100%. The mixture is uniformly grounded to obtain mixture of carbon paste. The carbon paste is placed into glass tube with wire with inner diameter of phi 4 mm, compacted, dried, polished with polishing powder, and washed with deionized water to obtain indium nitride paste electrode sensor. The 78-82 wt.% deionized water, 14-18 wt.% triethylenetetramine and 3-6 wt.% carbon quantum dots are added in reactor, stirred to dissolve, placed indium nitride paste electrode, and soaked at room temperature for 4 hours. The temperature rises to 45 plus minus 2 degrees C, and reacted at constant temperature for 1 hours. USE - Method for preparing sulfo-NHS-biotin modified indium nitride paste electrode sensor. ADVANTAGE - The method enables to prepare sulfo-NHS-biotin modified indium nitride paste electrode sensor that has higher conductivity than ordinary carbon paste electrodes and specific recognition of protein and high sensitivity. DETAILED DESCRIPTION - Preparing sulfabiotin modified indium nitride paste electrode sensor involves adding 48-52 wt.% nano indium nitride, 20-24 wt.% graphene oxide, 12-14 wt.% 1-aminopropyl-3-methylimidazole bromide, 6-8 wt.% mineral oil and 6-10 wt.% pentanol in agate mortar. The total mass percentage of each component is 100%. The mixture is uniformly grounded to obtain mixture of carbon paste. The carbon paste is placed into glass tube with wire with inner diameter of phi 4 mm, compacted, dried, polished with polishing powder, and washed with deionized water to obtain indium nitride paste electrode sensor. The 78-82 wt.% deionized water, 14-18 wt.% triethylenetetramine and 3-6 wt.% carbon quantum dots are added in reactor, stirred to dissolve, placed indium nitride paste electrode, and soaked at room temperature for 4 hours. The temperature rises to 45 plus minus 2 degrees C, and reacted at constant temperature for 1 hours. The electrode is removed, washed with deionized water and absolute ethanol, and dried to obtain triethylenetetramine/quantum dot modified indium nitride paste electrode. The 92-96 wt.% phosphate buffer solution and 4-8 wt.% sulfabiotin in reactor, and dissolved. The triethylenetetramine/quantum dot modified indium nitride paste electrode is placed into solution, stirred, and soaked for 2 hours at room temperature. The electrode is taken out, washed with phosphate buffer solution and absolute ethanol, and dried to obtain sulfabiotin modified indium nitride paste electrode sensor.