▎ 摘　　要

NOVELTY - Method for inhibiting peroxidase activity involves preparing graphene quantum dots, dispersing graphene quantum dots in double distilled water, adding graphene quantum dot solution and enzyme solution to buffer solution, adding coloring agent and hydrogen peroxide standard solution, vibrating, adding the solution to a cuvette, measuring the light absorption value at maximum absorption wavelength of the product using an ultraviolet-visible spectrophotometer, and performing blank sample control, such that the relative enzyme activity is determined. USE - Method for inhibiting peroxidase activity using graphene quantum dots (claimed). ADVANTAGE - The method enables inhibition of peroxidase activity using environmentally-friendly graphene quantum dots which are economically prepared. The structure of the enzyme is changed by the interaction between graphene quantum dots and enzyme protein, to regulate enzyme activity. DETAILED DESCRIPTION - Method for inhibiting peroxidase activity involves preparing graphene quantum dots, dispersing graphene quantum dots in double distilled water to stable graphene quantum dot solution with concentration of 10-10000 mu g/mL, adding predetermined amount of graphene quantum dot solution and enzyme solution to a buffer solution at room temperature for predetermined period of time, adding coloring agent and hydrogen peroxide standard solution, uniformly vibrating the mixed solution, rapidly adding the solution to a cuvette, measuring the light absorption value at maximum absorption wavelength of the product using an ultraviolet-visible spectrophotometer, and performing blank sample control, such that the relative enzyme activity is determined. The graphene quantum dots is prepared by adding predetermined amount of water to carbon source, or carbon source and surface passivation agent, mixing, heating mixed solution at predetermined temperature until water is completely evaporated in an autoclave, dissolving obtained product in predetermined amount of water, adjusting pH value to neutral with alkali, dialyzing with dialysis bag (molecular weight cut-off 1000 Da) and drying.