• 专利标题:   Method for preparing molecularly imprinted sensor, involves preparing nano silver-graphene oxide modified solution followed by preparing nano silver-graphene oxide modified electrode and adding molecularly imprinted polymer solution.
  • 专利号:   CN109001286-A
  • 发明人:   LI H, YANG Q, ZHANG Y
  • 专利权人:   UNIV JINAN
  • 国际专利分类:   G01N027/416, G01N027/30
  • 专利详细信息:   CN109001286-A 14 Dec 2018 G01N-027/416 201914 Pages: 7 Chinese
  • 申请详细信息:   CN109001286-A CN10995004 29 Aug 2018
  • 优先权号:   CN10995004

▎ 摘  要

NOVELTY - A molecularly imprinted sensor preparation method involves adding 5%, 86-92 mass% ammonia solution, 1-3 mass% silver nitrate and 2-6 mass% graphene oxide to reactor, ultrasonically processing at room temperature for 30 minutes, uniformly dispersing, adding 3-8% sucrose, reacting at room temperature for 20 minutes to obtain nano silver-graphene oxide modified solution followed by obtaining nano silver-graphene oxide modified electrode and molecularly imprinted polymer, adding appropriate amount of molecularly imprinted polymer to 1% chitosan-acetic acid solution, obtaining molecularly imprinted polymer solution, adding the solution (15-18 mu l) on the nano silver-graphene oxide modified electrode in dropwise, placing under infrared lamp and volatilizing solvent. USE - Method for preparing molecularly imprinted sensor. ADVANTAGE - The method enables preparing molecularly imprinted sensor with repeated usage property, high sensitivity and selectivity, better specificity and rapid detection property. DETAILED DESCRIPTION - A molecularly imprinted sensor preparation method involves adding 5%, 86-92 mass% ammonia solution, 1-3 mass% silver nitrate and 2-6 mass% graphene oxide to reactor, ultrasonically processing at room temperature for 30 minutes, uniformly dispersing, adding 3-8% sucrose, reacting at room temperature for 20 minutes to obtain nano silver-graphene oxide modified solution, utilizing 0.3 mu m glassy carbon electrode and 0.01 mu m polishing powder for polishing surface of the glassy carbon electrode, ultrasonically cleaning the electrode using double distilled water, washing using ethanol, drying, coating 15-18 mu l nano silver-graphene oxide modified solution on surface of the glassy carbon electrode, placing the electrode under infrared lamp, volatilizing solvent to obtain nano silver-graphene oxide modified electrode, adding 22-26 mass% N,N-dimethylformamide, 6mol/l, 38-43 mass% hydrochloric acid, 8-12 mass% o-aminophenol, 6-10 mass% 4-aminopyridine, 2-5 mass% oxadiazon, 1-2 mass% N,N-methylenebisacrylamide and 10%, 10-15 mass% ammonium persulfate to reactor, infusing nitrogen for 15 minutes, stirring at 40 plus minus 2 degrees C for 6-8 hours, under anaerobic conditions, performing solid-liquid separation, soaking obtained product in mixed solution comprising ethanol and hydrochloric acid in a volume ratio of 8:1 for 8 hours, washing several times, removing template molecule, drying to obtain molecularly imprinted polymer, adding appropriate amount of molecularly imprinted polymer to 1% chitosan-acetic acid solution, obtaining molecularly imprinted polymer solution, adding the solution (15-18 mu l) on the nano silver-graphene oxide modified electrode in dropwise, placing under infrared lamp and volatilizing solvent. An INDEPENDENT CLAIM is also included for a molecularly imprinted sensor prepared by the method.