▎ 摘　　要

NOVELTY - Method for preparing three-dimensional graphene biocomplex material self-assembled by Desulfovibrio vulgaris and its application, are claimed. The method involves (a) cultivating suspension of D.vulgaris, (b) preparing 2 g/l graphene oxide dispersion, (c) adding the graphene oxide dispersion to the anaerobic headspace flask containing lactic acid sulfate medium and mixing uniformly until the concentration reaches 1-8 g/l, and (d) adding 0.5 ml bacterial suspension to the headspace bottle, incubating at 37 degrees C for 12 hours, and in the solution of the anaerobic bottle, obtaining product. USE - The method is useful for preparing graphene biocomplex material self-assembled by D.vulgaris which is used for reducing and removing inorganic ionic contaminants in an aqueous solution and in preparation of a bioactive complex electrode in a bioelectrochemical system. The inorganic ion contaminants include sulfate ion and nitrite ion (all claimed). ADVANTAGE - The method prepares graphene biocomplex material having a large specific surface area and strong microbial activity. The graphene biocomplex material removes sulfate and nitrite in water. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following: (1) graphene biocomplex material self-assembled by D.vulgaris prepared by the above-mentioned method, where the graphene biocomplex material self-assembled by D.vulgaris is in the form of a cylinder or an irregular cluster, and the whole is loose and porous; and (2) use of the graphene biocomplex material self-assembled by D.vulgaris, for reducing and removing inorganic ionic pollutants in an aqueous solution.