▎ 摘　　要

NOVELTY - Method of fabricating biological field-effect transistor (BioFET) involves preparing carbonaceous dispersion by adding three-dimensional (3D) graphene into a solvent, depositing the carbonaceous dispersion onto a p-type silicon wafer, spin-coating a positive photoresist over the carbonaceous dispersion, forming source and drain terminals on the p-type silicon wafer, the source and drain terminals in contact with the 3D graphene of the carbonaceous dispersion, removing residual photoresist from the carbonaceous dispersion by placing the p-type silicon wafer in 1-methyl-2-pyrrolidone (NMP), and biofunctionalizing the carbonaceous dispersion with a molecular recognition element configured to alter one or more electrical properties of the Bio-FET in response to exposure of the molecular recognition element to the analyte. USE - The method is useful for fabricating BioFET or detecting harmful analytes. ADVANTAGE - The method and accurately detects the presence and concentration levels of analytes. The BioFET exhibits adverse ionic screening effects at physiologically relevant conditions. The pristine 3D graphene provides an improved surface area to volume ratio without suffering impediments resulting from impurities residing on exposed carbonaceous surfaces of the pristine 3D graphene.