▎ 摘　　要

NOVELTY - A boron-doped graphene quantum dot electrochemiluminescence sensor producing method involves preparing graphite oxide by adding concentrated sulfuric acid to graphite powder and adding potassium permanganate to the mixture. The mixture is separated by centrifugation, treated with hydrochloric acid and washed with water to obtain graphite oxide. Boron-doped graphene rod preparing process is performed. Gold nanoparticles aqueous solution is added to a platinum electrode surface, the surface is dried and boron-doped graphene quantum dots-DNA is added to platinum/gold electrode surface. USE - Method for producing boron-doped graphene quantum dot electrochemiluminescence sensor for detecting micro RNA-20a. ADVANTAGE - The method enables producing boron-doped graphene quantum dot electrochemiluminescence sensor with high practicality in cost-effective and easy manner. DETAILED DESCRIPTION - A boron-doped graphene quantum dot electrochemiluminescence sensor producing method involves preparing graphite oxide by adding concentrated sulfuric acid to graphite powder under stirring in ice bath and adding potassium permanganate to the mixture. The stirring is continued for 20-48 hours. Water is added and allowed to stand for 10- 60 minutes. Water and hydrogen peroxide are added to terminate the reaction. The mixture is separated by centrifugation, and treated with a concentration of 5% hydrochloric acid and washed with water to obtain graphite oxide, where the mole ratio of the concentrated sulfuric acid, graphite powder, potassium permanganate, water and hydrogen peroxide is 1:0.3:0.02:10:0.2. Boron-doped graphene rod preparing process is performed by adding obtained graphite oxide to boric acid mixture, heat preserving at 60-100 degrees C for 6-12 hours, washing the product with clean water, and vacuum freezing and drying product to obtain boron-doped graphene rod, where the mass ratio of the graphite oxide and boric acid is 250-5000. An aqueous solution of boron doped graphene quantum dot preparing process is performed by taking boron doped graphene rod as working electrode and graphite rod as counter electrode and performing electrochemical method for 1-3 hours under current density of 180-300 mA/cm2 in presence of electrolysis solution including a mixture containing 25-50 mM sodium hydroxide, absolute ethanol and water, where the volume ratio of ethanol and water is 99:1-99.5:0.5, centrifuging to remove graphite impurities, dialyzing the resulting centrifugate for 24-48 hours under aperture of less than 5000 Da to obtain an aqueous solution of boron doped graphene quantum dot. Electrochemiluminescent boron-doped graphene quantum dots-DNA probe preparing process is performed by adding sodium hydroxide and chloroacetic acid to the aqueous solution of boron-doped graphene quantum dot, adding 150 mg/ml N-hydroxysuccinimide and 10 mg/ml 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide, reacting for 10-60 minutes, adding 100 mu M hairpin DNA mercapto mark, slowly stirring, centrifuging mixture to obtain electrochemiluminescent boron-doped graphene quantum dots-DNA probe as precipitate, dispersing the probe in 0.1 M tris-EDTA buffer solution with a pH of 8 and storing at 4 degrees C, where the mass ratio of sodium hydroxide and chloroacetic acid is 0.25-0.5 and the volume ratio of N-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide is 120-1200. Boron-doped graphene quantum dot electrochemiluminescence sensor preparing process is performed by adding gold nanoparticles aqueous solution in platinum electrode surface, as platinum/gold surface, where the volume of the gold nanoparticles aqueous solution and platinum/gold electrode surface is 2-8 mu l/mm2, drying surface at room temperature, adding boron-doped graphene quantum dots-DNA to platinum/gold electrode surface, where the volume of boron-doped graphene quantum dots-DNA and platinum/gold electrode surface is 2-6 mu l/mm2 and washing the surface with 0.1 M phosphate buffer solution having a pH of 7.4 for 1-3 hours to obtain boron-doped graphene quantum dot electrochemiluminescence sensor.