▎ 摘　　要

NOVELTY - Detecting bacterial drug resistance involves using nano-hole detecting probe and bacterial biological identification combined with the generated complex of specific signal, and using quantitative detection of the bacterial biological identification to detect the bacterial growth. USE - The methods, complex, device and kit are useful for detecting bacterial drug resistance; for detecting carbapenem-resistant Klebsiella pneumoniae; and for detection of microbial resistance, where microorganism is a bacterium, specifically Klebsiella pneumoniae (all claimed). ADVANTAGE - The method is a more optimized and efficient method for detecting bacterial resistance. The method for rapid detection of bacterial drug resistance has a higher accuracy if it eliminates the cause of RNA degradation during sample storage or transfer, and thus has great potential application value in clinical rapid drug resistance detection. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: (1) a 16S rRNA-probe complex that is formed by annealing the 16S rRNA of probes A and B and Klebsiella pneumoniae, where sequences of the probes A and B are respectively SEQ ID NO. 1 and SEQ ID NO. 2; (2) a device for detecting carbapenem-resistant Klebsiella pneumoniae, comprising a nano-hole, a probe, Klebsiella pneumoniae RNA extraction reagent unit, and nano-hole electrophysiological signal detection unit; (3) a kit for detecting carbapenem-resistant Klebsiella pneumoniae, comprising nano-hole, probe and RNA extraction reagent; and (4) detecting carbapenem-resistant Klebsiella pneumoniae by using the device, which involves: (1) in the Klebsiella pneumoniae RNA extraction reagent unit, using TRIZOL to extract the total RNA of Klebsiella pneumoniae, then washing with ethanol, adding DEPC water/RNase water to dissolve and then adding RNase inhibitor for storage; (2) annealing the probes A and B and the sample stored in step (1) to form 16S rRNA-probe complex; (3) placing the nano-hole and the 16S rRNA-probe complex in the step (2) in a nano-hole electrophysiological signal detection unit for detection; and (4) performing data analysis on the detected electrophysiological signal; and quantitatively detecting Klebsiella pneumoniae. Sequences not defined here may be defined at ftp://ftp.wipo.int/pub/published_pct_sequences/publication.