▎ 摘　　要

NOVELTY - Substituted purin-6-one compounds (I) are new. USE - (I) are useful for identifying molecules for purifying molecules comprising a G-quadruplex and identifying molecules for in vitro and/or ex vivo imaging molecules comprising a G-quadruplex structure (all claimed). ADVANTAGE - (I): exhibits high affinity for G-quadruplex structures, in vitro and in vivo, and which are very versatile and can be substituted easily by functional residues; utilizes lanthanide lumiphores that have emission lifetimes in the millisecond range, hence robust and efficient; and contains a moiety that allows to carry click chemistry. DETAILED DESCRIPTION - Substituted purin-6-one compounds of formula (I) and their salts or solvates are new. A = absent or is a metallic cation, preferably a lanthanide used for optical imaging, or Eu3+, Tb3+, Dy3+, or Yb3+; X1, X2, X4 = 1-3C linear alkyl; X3 = 2-3C alkyl substituted by R1; Y1-Y4 = 1-10C alkyl, optionally saturated and optionally substituted by an acid moiety, possibly with a substitution of at least one C atom by N, P, phenyl, C=O, phosphonate or a triazole; Z1-Z4 = 1-5C linear alkyl; R1 = (CH2)p-L1-T1; p = 0-5; L1 = linear or branched 1-12C alkyl (optionally saturated, possibly with a substitution of at least one C by a C=O or an heteroatom), aryl (possibly substituted), triazole group or a diazirine; T1 = moiety allowing to carry out bioorthogonal reactions, preferably in azide, optionally substituted tetrazine, alkyne, constrained alkyne e.g. cyclooctyne or cyclononyne, preferably dibenzocyclooctyne, bicyclononyne, constrained cycloalkenes, e.g. trans-cyclooctene, norbornene and cycloproprene; and R2 = (CH2)mNHR1x; m = 1-4; and R1x = H or a protecting moiety e.g. Boc, Fmoc, Cbz, or guanidinium moiety. INDEPENDENT CLAIMS are also included for: (1) kit comprising the compound (I), and in association with a compatible compound allowing click chemistry; (2) identifying molecules for purifying molecules comprising a G quadruplex, comprising contacting a molecule to be identified/purified with the compound (I); and (3) identifying molecules for in vitro and/or ex vivo imaging molecules comprising a G quadruplex structure, comprising contacting a molecule comprising a G quadruplex structure to be identified with the compound (I), and coupling the compound with a fluorophore, a biotin derivative, solid or biological support.