▎ 摘　　要

NOVELTY - Preparing cell nucleus self-targeting fluorescent probe comprises (a) using 1,5-diaminonaphthalene as the precursor, ethanol and chloroform as a mixed solvent in a volume ratio of 1:1-3:2, and ultrasonically dispersing to obtain reaction liquid, (b) adding the reaction liquid to polytetrafluoroethylene reactor, and reacting at 230-250 degrees C for 10-20 hours, (c) naturally cooling to room temperature, then taking out, and filtering with 25 nm filter membrane to obtain black solution with a large number of stably dispersed graphene quantum dots, (d) transferring the filtered solution to a dialysis bag for dialysis, where the solvent is an aqueous solution during dialysis, and (e) using purified graphene quantum dot solution as a nucleus targeting fluorescent probe, and exciting at a wavelength of 633 nm for nuclear imaging. USE - The fluorescent probe is useful in the field of bioimaging technology, i.e. cell nuclear imaging. ADVANTAGE - The probe: is excited by 633 nm; and has non-toxic, fluorescently stable long-wavelength excitation and has autonomous nuclear targeting functions. The method: is simple, environmentally friendly, and is suitable for industrial amplification.