▎ 摘 要
NOVELTY - A nitrogen-doped graphene quantum dots fluorescence quenching method involves adding a series of bright blue standard solutions with increasing concentrations, adding the same amount of nitrogen-doped graphene quantum dots, setting the volume to the same volume and measuring the fluorescence intensity of the solution by a fluorescence spectrophotometer to obtain a linear relationship between the decrease in the fluorescence intensity of the graphene quantum dots and the concentration of bright blue and taking as working curve. The sample to be tested is added to the nitrogen-doped graphene quantum dot solution, determined the fluorescence intensity of the solution and determined the content of bright blue in the sample to be tested according to the regression equation of the working curve as given in the specification. USE - Method for fluorescence quenching of nitrogen-doped graphene quantum dots to detect bright blue content. ADVANTAGE - The method enables performing the fluorescence quenching of nitrogen-doped graphene quantum dots with high selectivity and high sensitivity, in simple and rapid manner. DETAILED DESCRIPTION - A nitrogen-doped graphene quantum dots fluorescence quenching method involves adding a series of bright blue standard solutions with increasing concentrations, adding the same amount of nitrogen-doped graphene quantum dots, setting the volume to the same volume and measuring the fluorescence intensity of the solution by a fluorescence spectrophotometer to obtain a linear relationship between the decrease in the fluorescence intensity of the graphene quantum dots and the concentration of bright blue and taking as working curve. The sample to be tested is added to the nitrogen-doped graphene quantum dot solution, determined the fluorescence intensity of the solution and determined the content of bright blue in the sample to be tested according to the regression equation of the working curve as given in the specification, where the concentration of bright blue is proportional to F0/F, where F0 is the fluorescence intensity of the nitrogen-doped graphene quantum dots added to the bright blue and F is the fluorescence intensity of the nitrogen-doped graphene quantum dots after the addition of bright blue.