• 专利标题:   Method for fluorescence quenching of nitrogen-doped graphene quantum dots to detect bright blue content, involves adding sample to be tested to nitrogen-doped graphene quantum dot solution, and determining fluorescence intensity of solution.
  • 专利号:   CN108872170-A
  • 发明人:   ZHANG J, JIN L, ZHANG Z
  • 专利权人:   JILIN INST CHEM TECHNOLOGY
  • 国际专利分类:   G01N021/64
  • 专利详细信息:   CN108872170-A 23 Nov 2018 G01N-021/64 201905 Pages: 5 Chinese
  • 申请详细信息:   CN108872170-A CN10616366 15 Jun 2018
  • 优先权号:   CN10616366

▎ 摘  要

NOVELTY - A nitrogen-doped graphene quantum dots fluorescence quenching method involves adding a series of bright blue standard solutions with increasing concentrations, adding the same amount of nitrogen-doped graphene quantum dots, setting the volume to the same volume and measuring the fluorescence intensity of the solution by a fluorescence spectrophotometer to obtain a linear relationship between the decrease in the fluorescence intensity of the graphene quantum dots and the concentration of bright blue and taking as working curve. The sample to be tested is added to the nitrogen-doped graphene quantum dot solution, determined the fluorescence intensity of the solution and determined the content of bright blue in the sample to be tested according to the regression equation of the working curve as given in the specification. USE - Method for fluorescence quenching of nitrogen-doped graphene quantum dots to detect bright blue content. ADVANTAGE - The method enables performing the fluorescence quenching of nitrogen-doped graphene quantum dots with high selectivity and high sensitivity, in simple and rapid manner. DETAILED DESCRIPTION - A nitrogen-doped graphene quantum dots fluorescence quenching method involves adding a series of bright blue standard solutions with increasing concentrations, adding the same amount of nitrogen-doped graphene quantum dots, setting the volume to the same volume and measuring the fluorescence intensity of the solution by a fluorescence spectrophotometer to obtain a linear relationship between the decrease in the fluorescence intensity of the graphene quantum dots and the concentration of bright blue and taking as working curve. The sample to be tested is added to the nitrogen-doped graphene quantum dot solution, determined the fluorescence intensity of the solution and determined the content of bright blue in the sample to be tested according to the regression equation of the working curve as given in the specification, where the concentration of bright blue is proportional to F0/F, where F0 is the fluorescence intensity of the nitrogen-doped graphene quantum dots added to the bright blue and F is the fluorescence intensity of the nitrogen-doped graphene quantum dots after the addition of bright blue.