▎ 摘　　要

NOVELTY - Metal nano-dot/single layer graphene surface-enhanced Raman spectroscopy (SERS) substrate comprises a silicon substrate and single-layer graphene and uniform metal nano-dot, where the single graphene layer is located on the silicon substrate, the uniform metal nano-dot is uniformly distributed on the single-layer graphene, and the uniform metal nano lattice is prepared by two-way nano hole array. USE - The metal nano-dot/single layer graphene SERS substrate is useful for detecting hydrophobic aromatic compound (claimed). ADVANTAGE - The graphene SERS substrate: is coupling the electromagnetic enhancement of metal nanostructures with the chemical enhancement of graphene; the preparation method: is simple and easy to operate; can prepare large area SERS substrates, and can uniformly distributes the hot spots on the surface of the prepared SERS substrate having large area; and improves SERS signal repeatability and stability. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for preparing the graphene SERS substrate, comprising (i) transferring a single-layer graphene grown by chemical vapor deposition (CVD) to a silicon substrate; (ii) transferring the obtained double-pass nano template to the single-layer graphene substrate obtained in the step (i); (iii) using the substrate of the transferred nano template to deposit nanostructure particles by physical vapor deposition, depositing metal nanostructure particles with Raman enhancement effect in the pores of a nanostructure template, and combining with the substrate obtained in the step (ii); and (iv) placing the formed substrate obtained in the step (iii) into a diluted solution, removing the nanostructure template.