▎ 摘　　要

NOVELTY - A pathogenic bacteria detecting method involves subjecting magnetic nanoparticles to surface carboxylation modification and reacting with antibacterial peptides to obtain antimicrobial peptide functionalized magnetic nanoparticles, modifying graphene oxide by thiolation, mixing with silver-coated gold nanoparticles to obtain a graphene oxide-silver-coated gold nanocomposite, mixing with p-nonylbenzeneboronic acid to obtain p-nonylbenzeneboronic acid-modified graphene oxide-silver-coated gold nanocomposite surface-enhanced Raman scattering probe, incubating antimicrobial peptide functionalized magnetic nanoparticles and bacteria, separating magnetic nanoparticles adsorbing the bacteria under the action of an external magnetic field to obtain a magnetic nanoparticle-bacteria complex, separating the sandwich structure under the action of an external magnetic field and identifying different bacteria by surface-enhanced Raman scattering. USE - Method for detecting pathogenic bacteria. DETAILED DESCRIPTION - A pathogenic bacteria detecting method involves subjecting magnetic nanoparticles to surface carboxylation modification and reacting with antibacterial peptides to obtain antimicrobial peptide functionalized magnetic nanoparticles, modifying graphene oxide by thiolation, mixing with silver-coated gold nanoparticles to obtain a graphene oxide-silver-coated gold nanocomposite, mixing with p-nonylbenzeneboronic acid to obtain p-nonylbenzeneboronic acid-modified graphene oxide-silver-coated gold nanocomposite surface-enhanced Raman scattering probe, incubating antimicrobial peptide functionalized magnetic nanoparticles and bacteria, separating magnetic nanoparticles adsorbing the bacteria under the action of an external magnetic field to obtain a magnetic nanoparticle-bacteria complex. The magnetic nanoparticle-bacterial complex is co-incubated with a non-p-phenylbenzeneboronic acid-modified graphene oxide-silver-coated gold nanocomposite surface-enhanced Raman scattering probe to form an antimicrobial peptide functionalized magnetic nanoparticle-bacteria-sandwich structure composed of surface-enhanced Raman scattering probes and separated the sandwich structure under the action of an external magnetic field, followed by identifying of different bacteria by surface-enhanced Raman scattering.