▎ 摘　　要

NOVELTY - Protein-containing collagen-based bone repair material comprises mesoporous hydroxyapatite, modified stone, polylactic acid glycolic acid copolymer, type I collagen bone repair material body, graphene oxide-polypyrrole self-assembled layer and bone morphogenetic protein-2 immobilized on the self-assembled layer. USE - Used as protein-containing collagen-based bone repair material. ADVANTAGE - The repair material has non-toxic and harmless raw materials, good biocompatibility, excellent mechanical properties, consistent degradation rate relative with the speed of bone defect repair, has strong osteogenic activity and forms a controlled release system of bone morphogenetic protein-2, no sudden release or no release, which improves the utilization of bone morphogenetic protein-2, and avoids complications caused by excessive bone morphogenetic protein-2 concentration. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for preparing protein-containing collagen-based bone repair material, comprising: (i) dissolving polylactic acid glycolic acid copolymer (PLGA) and type I collagen in hexafluoroisopropanol, preparing into a solution, taking hydroxyapatite and modified medical stone at a mass ratio of 4:1, uniformly mixing, placing in ampoule bottle, adding dioxane/deionized water (v:v 80:20) mixed solution, ultrasonically treating in 60 degrees C aqueous solution to uniform dispersion to obtain dispersion liquid; (ii) adding PLGA and type I collagen solution into the ampoule bottle containing the dispersion to obtain a mixed solution, taking out the ampoule bottle and placing at 80-90 degrees C, immersing in -50 degrees C ethanol solution for quick freezing molding, and maintaining for 2-4 hours, curing, breaking the ampoule bottle, removing the sample, and freeze-drying for more than 48 hours to obtain bone repair materials; (iii) immersing bone repair material in a polyethyleneimine solution for amination modification, taking out, washing, drying, immersing in hydrochloric acid solution, taking out, washing, and drying; (iv) immersing bone repair material modified by the triamination in graphene oxide dispersion liquid, self-assembling a layer of graphene oxide on surface, taking out, rinsing with water, placing in an electrode system of calcium nitrate solution electrolyte with pyrrole monomer, carrying out polypyrrole self-assembly under the effect of pulse voltage, and controlling the number of layers of polypyrrole by setting the number of pulses; and (v) washing the bone repair material with deionized water, vacuum drying, immersing in the bone morphology protein-2 solution, taking out bone morphogenetic protein-2 samples and drying in a 37 degrees C oven.