▎ 摘　　要

NOVELTY - Silver/graphene quantum dot nanozyme comprises a core-shell structure, the core in the core-shell structure comprises silver, the shell layer comprises graphene quantum dots, and the size of the silver/graphene quantum dot nanozyme is 7-15 nm, the core size is 6 to 14 nm, and the thickness of the shell layer is 1 to 3 nm. USE - Silver/graphene quantum dot nanozyme for making kit and detecting intracellular hydrogen peroxide, used in combination with SERS technology to realize the detection of antigen, polypeptide or DNA, and the nano-enzyme immunoassay kit is capable of detecting an antigen, a polypeptide or a DNA, preferably capable of specifically recognizing the detection of alpha-fetoprotein (all claimed). ADVANTAGE - The silver/graphene quantum dot nanozyme has good stability and SERS enhancement ability, and the silver/graphene quantum dot nanozyme is used as the SERS substrate, and the PATP is the probe molecule for the SERS test, which remains good for 16 months when the probe molecule is subjected to SERS. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: (1) a method for preparing a silver/graphene quantum dot nanozyme, which involves forming a homogeneous mixed reaction system comprising graphene quantum dots and a silver salt in an alkaline environment at 100-150 degrees C degree for 0.5-1.5 hours, and then the silver/graphene quantum dot nanozyme is obtained by centrifugal washing; (2) an intracellular in situ SERS detection silver/graphene quantum dot nanozyme catalytic kit, which comprises the silver/graphene quantum dot nanozyme; (3) a method for detecting intracellular hydrogen peroxide by in situ SERS, which involves using silver/graphene quantum dot nanozyme as catalyst to monitor the oxidation of chromogenic substrate TMB by silver/graphene quantum dot nano-enzyme in situ in the presence of different concentrations of hydrogen peroxide; and detecting the signal of oxidized TMB in the cells in situ by SERS, and the concentration of hydrogen peroxide in the cells and cells is detected in comparison with the SERS spectra in vitro and in the cells; (4) an in situ SERS detection silver/graphene quantum dot nano-enzyme immunoassay kit, which comprises the silver/graphene quantum dot nanozyme; and (5) a method of detecting an antigen, polypeptide or DNA carried out by the nano-enzyme immunoassay kit, for detecting alpha-fetoprotein, which involves: labeling silver/graphene quantum dot nanozyme with alpha-fetoprotein antibody; obtaining a silver/graphene quantum dot nanozyme-labeled alpha-fetoprotein antibody; combining the silver/graphene quantum dot nanozyme-labeled alpha-fetoprotein antibody with the TMB-H2O2 system for SERS detection; and combining the silver/graphene quantum dot nanozyme-labeled alpha-fetoprotein antibody with the alpha-fetoprotein antigen to obtain an alpha-fetoprotein antigen-silver/graphene quantum dot nano-enzyme-labeled alpha-fetoprotein antibody complex, and then the TMB-hydrogen peroxide system is combined to perform SERS detection, and the results of two SERS tests are compared to achieve the detection of alpha-fetoprotein.