▎ 摘　　要

NOVELTY - Performing impedance fast detection process of Escherichia coli in food based on nano-magnetic beads involves obtaining graphene paper flexible electrode through vacuum filtration and reduction and performing nano-particle and polymer modification on the electrode surface to obtain the graphene/nanocomposite paper electrode. The surface of the graphene/nano-composite paper electrode was modified with colon Bacillus identification function of the element. The corresponding nano-magnetic bead is synthesized and the identification element is modified with colon Bacillus to capture the surface of the magnetic bead. The immunomagnetic beads capture bacteria and combine with the antibody on the electrode surface to form a sandwich structure and generate an impedance signal. The linear relationship is analyzed between the impedance value and the added Escherichia coli concentration to obtain the correction equation of the electrode for Escherichia coli. USE - Method for performing impedance fast detection process of Escherichia coli in food based on nano-magnetic beads. ADVANTAGE - The method prepared impedance biological sensor detection sensitivity is higher, the detection result is more stable, the detection range is wider, with wide application prospects, and it can obtain great economic benefit.