▎ 摘　　要

NOVELTY - Preparing defective N-carbon nanotubes/carbon nanorods (N-CNTs/CNRs) composite material involves dispersing multi-walled carbon nanotubes in concentrated sulfuric acid, and adding potassium permanganate in ice-water bath. The obtained mixture is heated to 55-75degrees Celsius for 10-30 minutes, quenched, and allowed to stand at room temperature for 12-48 hours. The obtained mixture is centrifuged, and obtained product is lyophilized to obtain carbon nanotube/oxygen-graphene (CNT/O-Gr) precursor. The CNT/O-Gr precursor is dispersed in water, urea is added, placed in autoclave for hydrothermal reaction, and resulting product is dried to obtain N-CNTs/CNRs. The N-CNTs/CNRs is thermally annealed in an inert atmosphere to obtain defective N-CNTs/CNRs composite material. USE - Method for preparing defective N-carbon nanotubes/carbon nanorods (N-CNTs/CNRs) composite material for use as supporting material in preparation of bioanodes, biocathodes and non-membrane lactic acid biofuel cells (all claimed), which is used to constuct high performance biological electrode for biological electronic application and has application in wearable device. ADVANTAGE - The method provides high performance non-membrane lactic acid/oxygen biological fuel cell with enzyme and medium double fixed on the N-CNTs/CNRs, with good biological electro-catalysis performance. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following: (1) an application of defective N-CNTs/CNRs composite material, which is used as a supporting material in preparation of bioanodes, biocathodes and biofuel cells; (2) a method for preparing bioanode, which involves dropping mixed liquid of tetrathiafulvalene, lactic acid oxidase, and defective N-CNTs/CNRs composite material onto conductive electrode, and drying; (3) a method for preparing biocathode, which involves adding mixture of defective N-CNTs/CNRs composite material and 2,2'-diazide-bis-3-ethylbenzothiazolin-6-sulfonic acid dropwise to conductive electrode, drying, casting solution of bilirubin oxidase to obtain biocathode; and (4) a non-membrane lactic acid biofuel cell, which comprises anode of non-membrane lactic acid biofuel cell is bioanode, cathode is biocathode, and electrolyte is a buffer solution containing lactic acid.