▎ 摘　　要

NOVELTY - Method for preparing an integrated hydrogel for high altitude medical diagnosis and treatment, involves (a) dissolving and dispersing calcium chloride and dopamine-reduced graphene oxide in an organic solvent, and uniformly stirring at a certain temperature, adding 30 %mass hydrogen peroxide to the obtained mixture, subsequently dripping 1 mol/l aqueous ammonia, allowing the mixture to react and stir for 8 hours, and centrifuging the mixture to obtain calcium oxide-dopamine-reduced graphene oxide nanosheets, and (b) adding the calcium oxide-dopamine-reduced graphene oxide nanosheets to a glycerol/phosphate buffered saline mixed solution for uniformly dispersing, adding acrylamide and polysaccharides, and then adding initiator and crosslinking agent to obtain the product. USE - The method is used for preparing an integrated hydrogel, which is used in the skin tissue repair of the body, in the electrophysiological monitoring of the electromyography of the body in the plateau environment, and in the real-time and stable monitoring of physiological signals of the body (claimed), preferably in the tissue repair and electromyography or cardiac electrophysiological conduction monitoring in high altitude environment. ADVANTAGE - The method provides the hydrogel with anti-freezing ability in the plateau low temperature environment by using the glycerol system. The method provides the hydrogel with the ability to release oxygen in the plateau hypoxic environment by using the nanosheets, which can further promote the release of oxygen under the action of photothermal effect. The method provides the hydrogel with electrical conductivity, adhesion, and tissue affinity by using the nanosheets. DETAILED DESCRIPTION - Method for preparing an integrated hydrogel for high altitude medical diagnosis and treatment, involves (a) dissolving and dispersing calcium chloride and dopamine-reduced graphene oxide in an organic solvent, and uniformly stirring at a certain temperature, adding 30 %mass hydrogen peroxide to the obtained mixture, subsequently dripping 1 mol/l aqueous ammonia, allowing the mixture to react and stir for 8 hours, and centrifuging the mixture to obtain calcium oxide-dopamine-reduced graphene oxide nanosheets, and (b) adding the calcium oxide-dopamine-reduced graphene oxide nanosheets to a glycerol/phosphate buffered saline mixed solution for uniformly dispersing, adding acrylamide and polysaccharides, and then adding initiator and crosslinking agent to obtain the product. The organic solvent is methanol or ethanol. The dispersing process in step (a) involves ultrasonically dispersing for 5-10 minutes. The temperature condition in step (a) is 0-4degrees Celsius ice bath. The mass ratio of 30% hydrogen peroxide and calcium chloride is 5 ml:1 g. The mass ratio of 1 mol/l aqueous ammonia and calcium chloride is 5 ml:1 g. The mixed solution in step (b) comprises glycerol/phosphate buffered saline in a ratio of 1: 4, 1:3, or 1:2. The uniform dispersion process in step (b) involves ultrasonically dispersing for 1-2 minutes.