▎ 摘　　要

NOVELTY - Method for preparing modified graphene immobilized microorganism composite material, involves the alcaligenes and cationic polyacrylamide modified oxidized graphene are dispersed in PBS, immobilized treatment, centrifuging, freezing, dry the modified oxidized graphene immobilized microorganism composite material. USE - Preparation method of modified oxidized graphene immobilized microorganism composite material used for repairing diesel oil polluted soil in situ, belonging to the technical field of microbial repair. ADVANTAGE - The method: enables to prepare modified oxidized graphene immobilized microorganism composite material, which is not limited by environmental conditions, and has good application prospect and industrial production potential in the field of environment treatment method. The modified oxidation graphene has high carrier activity, the immobilized cell density is large, the graphene oxide has good mechanical strength and chemical stability, and it is resistant to microbial decomposition. The graphene oxide is easy to operate and easy to form. The diffusion resistance of the substrate and the product is small, it has good transmission performance, the activity recovery rate of the microorganisms is high, it can be used for a long time and used repeatedly, and the oxidation graphene surface a large amount of carboxyl functional group can be covalently bound with the amino of the bacteria surface. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: (1) a modified oxidized graphene immobilized microorganism composite material prepared by the preparation method of modified oxidized graphene immobilized microorganism composite material; (2) an application of the modified oxidized graphene immobilized microorganism composite material; and (3) a in-situ repairing method of diesel oil polluted soil. DESCRIPTION OF DRAWING(S) - The drawing shows a scanning electron microscope photograph of the preparation of modified oxidized graphene immobilized microorganism composite material.