▎ 摘　　要

NOVELTY - Method for preparing a zinc oxide/graphene quantum dot-polyethylenimine complex antibacterial agent involves taking 1-10 g citric acid (CA) into a beaker, heating the material to obtain orange liquid, adding 0.05-0.2 g 1-ethyl-(3-dimethylaminopropyl)carbonyldiimide (EDC) hydrochloride, ultrasonically treating for 20 minutes, adding 0.2-0.5 g EDC-hydrochloride ultrasonically treating for 20 minutes and stirring at room temperature for 24 hours under magnetic force and adding 0.2-1 g alkali source to 50 ml ethanol solution, magnetically stirring for 30 minutes to obtain solution (A), adding 0.5-2 g zinc source to 100 ml ethanol solution, magnetically stirring for 30 minutes to obtain solution (B), adding GQD-PEI obtained in the step (b) to the solution (B), ultrasonically treating for 10 minutes, adding solution (A) to solution (B), transferring the solution to a three-necked flask for heating in a water bath, cooling the solution, washing and centrifuging the solution. USE - The method is useful for preparing ZnO/GQD-PEI complex antibacterial agent. ADVANTAGE - The method is environmentally-friendly, and the prepared agent exhibits strong resistance and inhibits the Escherichia coli to 1.8 mg/ml compared with the traditional antibacterial agent. DETAILED DESCRIPTION - Method for preparing a zinc oxide/graphene quantum dot-polyethylenimine (ZnO/GQD-PEI) complex antibacterial agent involves (a) taking 1-10 g citric acid (CA) into a beaker, heating the material to obtain orange liquid, adding sodium hydroxide solution to adjust pH to 7 by dialysis and performing rotary evaporation, (b) diluting the GQDs obtained in the step (a) to 0.2-2 mg/ml with deionized water, adding 5-15 mg/ml modifier, ultrasonically treating for 10 minutes, adding 0.05-0.2 g 1-ethyl-(3-dimethylaminopropyl)carbonyldiimide hydrochloride (EDC-HCl), ultrasonically treating for 20 minutes, adding 0.2-0.5 g EDC-HCl ultrasonically treating for 20 minutes and stirring at room temperature for 24 hours under magnetic force and (c) adding 0.2-1 g alkali source to 50 ml ethanol solution, magnetically stirring for 30 minutes to obtain solution (A), adding 0.5-2 g zinc source to 100 ml ethanol solution, magnetically stirring for 30 minutes to obtain solution (B), adding GQD-PEI obtained in the step (b) to the solution (B), ultrasonically treating for 10 minutes, adding solution (A) to solution (B), transferring the solution to a three-necked flask for heating in a water bath, cooling the solution, washing and centrifuging the solution.