▎ 摘　　要

NOVELTY - Preparing dual-photon signal amplification probe based on aptamer-modified silica-graphene quantum dots, is claimed. The dual-photon signal amplification probe based on aptamer-modified silica-graphene quantum dots comprises graphene quantum dots, silica nanoparticles and aptamer Sgc8c, the graphene quantum dots is used as two-photon fluorescent signal units, which is prepared by N,N-dimethylformamide solvothermal method with graphene oxide as a carbon source, the silica nanoparticles as signal amplification carrier of functional nanomaterials, and is prepared by inverse microemulsion method, and through covalent bonding with graphene quantum dots to prepare silica-graphene quantum dot composite nanoparticles, the CCRF-CEM cell-specific aptamer Sgc8c is cross-linked and grafted on surface of silica-graphene quantum dot composite nanoparticles to prepare targeted composite dual-photon nanoprobe silicon dioxide-GQDs-Sgc8c. USE - The method is useful for preparing dual-photon signal amplification probe based on aptamer-modified silica-graphene quantum dots in specific recognition and high-sensitivity real-time imaging of CCRF-CEM target cells (claimed). ADVANTAGE - The method: has easy purification and separation; provides a one-to-many signal transduction mode for specific recognition of target cells and high-sensitivity real-time imaging detection.